Description
Specifications Table
| Product Name | Biolit? 10x Pcr Buffer With Kcl (Mg?? Free) | 61318 | SRL Chem |
|---|---|
| Pack Size | 4 × 1.25 ml |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | PCR, DNA amplification, molecular cloning |
Product Description
When it comes to reliable PCR amplification, the right buffer can make all the difference. The Biolit 10X PCR Buffer with KCl (Mg²⁺ Free) from SRL Chem is designed to deliver consistent results in molecular biology applications. Whether you’re a student setting up your first PCR experiment or a researcher optimizing reaction conditions, this buffer provides the stability and performance you need.
This Mg²⁺-free PCR buffer is formulated to support high-fidelity DNA amplification, making it a staple in labs across India. The inclusion of KCl helps maintain optimal ionic strength, ensuring efficient primer annealing and enzyme activity. Since it lacks magnesium, you have full control over Mg²⁺ concentrations, allowing for customization based on your specific assay requirements.
One of the key advantages of this 10X PCR buffer is its versatility. It works seamlessly with a wide range of DNA polymerases, including Taq and high-fidelity enzymes. This makes it an excellent choice for both routine PCR and more specialized applications like cloning, genotyping, and site-directed mutagenesis.
At eqipped, we understand the importance of quality reagents in achieving reproducible results. That’s why we offer this Biolit PCR buffer with the assurance of SRL Chem’s rigorous quality standards. Each batch undergoes strict quality control to ensure purity, consistency, and performance.
Need a dependable PCR buffer for your next experiment? Shop now and experience the difference with eqipped.
For researchers working on sensitive applications, the absence of magnesium in this buffer is particularly beneficial. It allows you to fine-tune Mg²⁺ levels, which is crucial for avoiding non-specific amplification and ensuring high yield. The buffer’s compatibility with various additives, such as DMSO or betaine, further enhances its utility in challenging PCR setups.
Students and educators will appreciate how this buffer simplifies PCR troubleshooting. Its standardized formulation reduces variability, making it easier to teach and learn PCR techniques without unexpected failures. Whether you’re demonstrating basic PCR principles or conducting advanced research, this buffer is a reliable partner.
When you buy PCR buffer online from eqipped, you’re not just purchasing a reagent—you’re investing in peace of mind. Our streamlined ordering process and fast delivery ensure that your lab stays stocked with essential supplies, so you can focus on what matters most: your research.
FAQs
What is the difference between this buffer and a standard PCR buffer?
This Biolit 10X PCR buffer is Mg²⁺-free, allowing you to adjust magnesium concentrations separately. Standard buffers often include Mg²⁺, which may not be ideal for all applications.
Can I use this buffer with any DNA polymerase?
Yes, this buffer is compatible with most DNA polymerases, including Taq and high-fidelity enzymes. However, always check the manufacturer’s recommendations for optimal performance.
How should I determine the right Mg²⁺ concentration for my PCR?
Start with a final concentration of 1.5–2.5 mM Mg²⁺ and optimize based on your specific primers and template. A gradient PCR can help identify the ideal concentration.
Is this buffer suitable for qPCR?
While it can be used for some qPCR applications, it’s best to use a buffer specifically designed for real-time PCR to ensure accurate and reproducible results.
Can I store this buffer at 4°C instead of -20°C?
For short-term storage (up to a few weeks), 4°C is acceptable. However, for long-term stability, -20°C is recommended to prevent degradation.
