Description
Specifications Table
| Product Name | Biolit™ 10x PCR Buffer with Mg²⁺ Set |
|---|---|
| Pack Size | 6 × 1.25 ml |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | PCR amplification, cloning, molecular diagnostics |
Product Description
When it comes to PCR, the right buffer can make all the difference. The Biolit™ 10x PCR Buffer with Mg²⁺ Set is a trusted choice for researchers, students, and educators who need reliable amplification every time. This buffer is pre-optimized with magnesium ions, ensuring consistent performance across a wide range of templates and primers.
Whether you’re working on academic projects, diagnostic assays, or cloning experiments, this buffer simplifies your workflow. No more guesswork—just add your template, primers, and polymerase, and let the buffer do the rest. The 6 × 1.25 ml pack size is perfect for labs with moderate throughput, reducing the need for frequent reorders.
At eqipped, we understand the importance of precision in molecular biology. That’s why we stock only high-quality reagents like this Biolit™ PCR buffer, so you can focus on your research without worrying about inconsistencies.
Need a dependable PCR buffer for your experiments? Shop now and experience seamless amplification.
Why This PCR Buffer Stands Out
Not all PCR buffers are created equal. This 10x PCR buffer with Mg²⁺ is formulated to enhance enzyme stability and primer binding, reducing the risk of failed reactions. The included magnesium ions eliminate the need for separate titration, saving you time and effort.
Students and educators will appreciate its ease of use, while researchers will value its reproducibility. Whether you’re amplifying DNA for sequencing, cloning, or diagnostic purposes, this buffer delivers consistent results batch after batch.
Applications in the Lab
This versatile buffer is suitable for:
- Standard PCR amplification
- High-fidelity PCR for cloning
- Diagnostic and forensic DNA analysis
- Academic research and training
Its compatibility with most Taq polymerases makes it a staple in any molecular biology lab.
Storage and Handling
Store the buffer at -20°C for long-term stability. Avoid repeated freeze-thaw cycles to maintain performance. Always use sterile techniques to prevent contamination.
FAQs
What is the concentration of Mg²⁺ in this buffer?
The buffer contains pre-optimized magnesium ions, typically around 15-20 mM in the 1x working concentration.
Can I use this buffer with other polymerases?
Yes, it is compatible with most standard Taq DNA polymerases. For high-fidelity enzymes, check the manufacturer’s recommendations.
How should I adjust the Mg²⁺ concentration if needed?
While the buffer is pre-optimized, you can supplement with additional MgCl₂ if required for specific templates.
Is this buffer suitable for gradient PCR?
Absolutely. Its stable formulation ensures reliable performance across a range of annealing temperatures.
Where can I buy Biolit™ 10x PCR Buffer with Mg²⁺ online?
You can order it directly from eqipped for fast delivery across India.
Risk Note
This product is for laboratory use only. Avoid skin contact and inhalation. Wear appropriate PPE when handling.
