Description
Specifications Table
| Product Name | Biolit® Rnasin (RNase Inhibitor) (40 U/µL) |
|---|---|
| Quantity/Pack Size | 1000 units |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | PCR, RT-PCR, cDNA synthesis, in vitro transcription |
Product Description
When working with RNA, even the slightest contamination can compromise your experiments. Biolit® Rnasin (RNase Inhibitor) is a highly effective protein-based inhibitor designed to protect RNA from degradation during sensitive molecular biology procedures. Whether you’re running PCR, RT-PCR, or synthesizing cDNA, this RNase inhibitor ensures your RNA remains intact for accurate and reproducible results.
This RNase inhibitor binds non-covalently to RNase A, B, and C, preventing them from degrading RNA. With a concentration of 40 U/µL, it provides robust protection without interfering with enzymatic reactions. Researchers and students alike trust Biolit® Rnasin for its consistency and reliability in critical applications.
If you’re looking to buy RNase inhibitor online, eqipped offers authentic Biolit® Rnasin from SRL Chem, ensuring you get a product that meets the highest standards of purity and performance.
Why Use Biolit® Rnasin in Your Experiments?
RNA is inherently unstable, and even trace amounts of RNases can lead to failed experiments. Biolit® Rnasin acts as a safeguard, allowing you to work with confidence. Here’s how it helps:
In PCR and RT-PCR, RNA integrity is crucial for accurate amplification. This inhibitor prevents RNA degradation during reverse transcription, ensuring your cDNA synthesis is efficient and error-free. For in vitro transcription, it maintains RNA stability, leading to higher yields of full-length transcripts.
Unlike some chemical inhibitors, Biolit® Rnasin is easily inactivated by heat, making it compatible with downstream applications. Its high specificity means it won’t interfere with other enzymes in your reaction mix.
Applications of Biolit® Rnasin (RNase Inhibitor)
This versatile RNase inhibitor is essential for a wide range of molecular biology techniques, including:
• Reverse transcription PCR (RT-PCR) – Protects RNA templates during cDNA synthesis.
• Quantitative PCR (qPCR) – Ensures accurate quantification by preventing RNA degradation.
• In vitro transcription – Maintains RNA stability for high-yield transcription reactions.
• RNA sequencing (RNA-Seq) – Preserves RNA quality for reliable sequencing results.
• cDNA library construction – Safeguards RNA during first-strand synthesis.
Whether you’re a student learning molecular techniques or a researcher conducting high-throughput experiments, Biolit® Rnasin is a must-have reagent in your lab.
How to Use Biolit® Rnasin Effectively
To get the best results, follow these guidelines when using this RNase inhibitor:
• Add Biolit® Rnasin to your reaction mix before introducing RNA to prevent degradation from the start.
• Use 1 unit of inhibitor per 10–20 units of RNase activity. For most applications, 1–2 µL per 50 µL reaction is sufficient.
• Store the inhibitor at -20°C to maintain its activity. Avoid repeated freeze-thaw cycles.
• If heating is required for downstream steps, the inhibitor can be inactivated at 65°C for 10 minutes.
Always handle RNA with care, using RNase-free tubes and tips to minimize contamination. Biolit® Rnasin works best when combined with good lab practices.
Choosing the Right RNase Inhibitor for Your Needs
Not all RNase inhibitors are created equal. Biolit® Rnasin stands out because of its high specificity and effectiveness. When selecting an RNase inhibitor, consider the following:
• Purity – Molecular biology grade ensures no interference with your experiments.
• Concentration – A 40 U/µL formulation provides flexibility in dosing.
• Compatibility – Works seamlessly with reverse transcriptases and polymerases.
• Stability – Remains active under typical reaction conditions.
If you’re unsure which RNase inhibitor to buy, Biolit® Rnasin is a trusted choice for both academic and industrial labs. Its proven performance makes it a staple in RNA-based workflows.
Where to Buy RNase Inhibitor Online in India
Finding a reliable supplier for lab reagents is crucial for consistent results. eqipped offers genuine Biolit® Rnasin from SRL Chem, ensuring you receive a product that meets stringent quality standards. When you buy RNase inhibitor online from eqipped, you benefit from:
• Authentic products sourced directly from manufacturers.
• Competitive pricing with transparent RNase inhibitor price listings.
• Fast and secure delivery across India.
• Dedicated support for any technical or ordering queries.
Whether you need RNase inhibitor for PCR, RT-PCR, or other applications, eqipped makes it easy to order online with just a few clicks.
FAQs
What is the role of RNase inhibitor in PCR?
RNase inhibitor prevents RNA degradation during reverse transcription in RT-PCR, ensuring accurate cDNA synthesis for reliable amplification.
Can Biolit® Rnasin be used in qPCR?
Yes, it is compatible with qPCR and helps maintain RNA integrity for precise quantification of gene expression.
How should I store Biolit® Rnasin?
Store at -20°C in RNase-free conditions. Avoid repeated freeze-thaw cycles to preserve activity.
Is this RNase inhibitor suitable for in vitro transcription?
Absolutely. Biolit® Rnasin protects RNA templates during in vitro transcription, improving yield and quality.
Where can I buy authentic RNase inhibitor online in India?
You can purchase Biolit® Rnasin from eqipped, a trusted supplier of lab reagents with fast delivery and competitive pricing.
