Description
Specifications Table
| Product Name | 5,5′-Dithiobis(2-Nitro Benzoic Acid) extrapure (DTNB), 98% (Ellman’s Reagent) |
|---|---|
| Pack Size | 5 Gms, 25 Gms, 100 Gms |
| Form | Solid |
| Grade | Extrapure |
| Application | Thiol quantification, biochemical assays, protein analysis |
Product Description
When precision matters in your lab, 5,5′-Dithiobis(2-Nitro Benzoic Acid) (DTNB), commonly known as Ellman’s Reagent, is the gold standard for quantifying thiol groups in proteins, peptides, and other biomolecules. This extrapure grade reagent, with a purity of 98%, ensures reliable and reproducible results in your research or diagnostic applications.
DTNB works by reacting with free thiol groups to produce a yellow-colored anion (TNB²⁻), which can be easily measured spectrophotometrically at 412 nm. Whether you’re studying enzyme kinetics, protein folding, or oxidative stress, this reagent provides the accuracy you need. Researchers and educators across India trust DTNB for its consistency and high sensitivity in detecting sulfhydryl groups.
At eqipped, we understand the importance of quality reagents in your experiments. That’s why we source DTNB from trusted suppliers like SRL Chem, ensuring you get a product that meets rigorous laboratory standards. Whether you’re a student setting up your first assay or a seasoned researcher optimizing protocols, this reagent delivers performance you can rely on.
Need DTNB for your next experiment? Buy Ellman’s reagent online today and experience seamless lab supply delivery.
The extrapure grade of this DTNB reagent minimizes impurities, reducing background interference in your assays. It’s particularly useful in applications like:
- Quantifying glutathione and other low-molecular-weight thiols
- Monitoring protein denaturation and folding
- Assessing oxidative damage in biological samples
- Validating thiol-containing pharmaceuticals
For best results, store the reagent in a cool, dry place away from direct light. Always handle with appropriate lab safety measures, as DTNB is a potent reagent that requires careful use.
FAQs
What is DTNB used for in laboratories?
DTNB, or Ellman’s Reagent, is primarily used to quantify thiol groups in proteins and other biomolecules. It’s essential in biochemical assays, enzyme kinetics studies, and protein structure analysis.
How do I prepare a DTNB solution for my assay?
Dissolve DTNB in a suitable buffer (e.g., 0.1 M sodium phosphate, pH 8.0) to a concentration of 1-10 mM. Filter if necessary and use immediately for best results.
What is the shelf life of DTNB reagent?
When stored properly in a desiccator at 2-8°C, DTNB has a shelf life of 2-3 years. Always check for discoloration before use, as degraded reagent may give inaccurate results.
Can I use DTNB for glutathione quantification?
Yes, DTNB is commonly used to measure glutathione levels in biological samples due to its high sensitivity and specificity for thiol groups.
Where can I buy authentic Ellman’s reagent in India?
You can buy DTNB 98% pure online from eqipped, where we offer genuine SRL Chem products with fast delivery across India.
