Description
Specifications Table
| Product Name | Ethylene Glycol-O-O-bis-(2-Aminoethyl) N,N,N,N-Tetraacetic Acid (EGTA) extrapure, 98% |
|---|---|
| Quantity/Pack Size | 10 Gms – 25 Gms – 100 Gms |
| Form | Powder |
| Grade | Extrapure |
| Application | Biochemical research, calcium ion chelation |
Product Description
EGTA extrapure 98% is a specialized chelating agent widely used in laboratories for its high affinity to calcium ions. Unlike EDTA, EGTA selectively binds calcium over magnesium, making it indispensable in biochemical assays, cell culture studies, and enzyme activity research. This high-purity grade ensures minimal interference in sensitive experiments, delivering reliable results every time.
Whether you’re studying intracellular calcium signaling or optimizing enzyme reactions, EGTA extrapure 98% provides the precision your research demands. Its extrapure grade guarantees low impurity levels, reducing background noise in assays. Researchers across India trust this chemical for its consistency and performance in critical applications.
At eqipped, we source EGTA directly from trusted suppliers like SRL Chem to ensure authenticity and purity. Our streamlined ordering process makes it easy to buy EGTA online, with fast shipping to labs, universities, and research institutions nationwide. Need EGTA for your next experiment? Order now and experience hassle-free delivery.
Key Applications of EGTA Extrapure 98%
EGTA’s unique properties make it a staple in modern laboratories. Here’s how researchers leverage its capabilities:
In calcium-dependent enzyme studies, EGTA helps isolate the role of calcium ions by selectively chelating them without affecting magnesium. This is crucial in experiments involving ATPases, proteases, and other metalloenzymes. Cell biologists rely on EGTA to manipulate intracellular calcium levels, allowing precise control over cellular processes like apoptosis or muscle contraction.
Molecular biologists use EGTA in buffer solutions to prevent calcium-mediated degradation of DNA or proteins during extraction and purification. Its stability across a wide pH range makes it versatile for various protocols. Whether you’re running a Western blot or a PCR, EGTA ensures your samples remain intact.
For educators, EGTA serves as an excellent teaching tool in biochemistry labs. Demonstrating chelation principles becomes more effective with a compound that offers such specific binding properties. Students gain hands-on experience with a chemical that’s both theoretically significant and practically relevant.
Why EGTA Over Other Chelating Agents?
While EDTA is a common chelator, EGTA offers distinct advantages in certain scenarios. Its higher affinity for calcium (Kd ~ 10^-11 M) compared to magnesium (Kd ~ 10^-5 M) allows researchers to target calcium ions specifically. This selectivity is invaluable in experiments where magnesium ions must remain unchelated, such as in ATP-dependent reactions.
EGTA also exhibits better stability in alkaline conditions, making it suitable for experiments requiring higher pH levels. Its slower binding kinetics compared to EDTA provide more controlled chelation, which is beneficial in time-sensitive assays. These properties make EGTA the preferred choice for researchers who need precision in their calcium manipulation.
Safety and Handling Guidelines
EGTA extrapure 98% is generally safe when handled properly, but standard laboratory precautions should be followed. Always wear appropriate personal protective equipment (PPE), including gloves and safety goggles, when handling the powder. Avoid inhalation and ensure proper ventilation in the workspace.
Store EGTA in a cool, dry place away from direct sunlight and moisture. Keep the container tightly sealed to prevent contamination. For disposal, follow local regulations for chemical waste to ensure environmental safety.
FAQs
What is the difference between EGTA and EDTA?
EGTA selectively binds calcium ions, while EDTA chelates a broader range of metal ions, including magnesium. EGTA is preferred when calcium-specific chelation is required.
Can EGTA be used in cell culture media?
Yes, EGTA is commonly added to cell culture media to study calcium-dependent cellular processes. However, optimal concentrations should be determined experimentally.
What is the solubility of EGTA extrapure 98%?
EGTA is soluble in water and slightly soluble in ethanol. For most lab applications, preparing a stock solution in deionized water is recommended.
Is EGTA suitable for protein purification?
Yes, EGTA is often used in protein purification buffers to prevent calcium-mediated proteolysis, ensuring protein integrity during isolation.
How should EGTA be stored for long-term use?
Store EGTA in a desiccator or airtight container at room temperature. Avoid exposure to humidity to maintain its purity and effectiveness.
