Description
Specifications Table
| Product Name | HEPES Buffer extrapure, 99% |
|---|---|
| Quantity/Pack Size | 25 Gms | 100 Gms | 500 Gms | 1 Kg |
| Form | Powder |
| Grade | Extrapure |
| Application | Cell culture, biochemical assays, pH buffering |
Product Description
HEPES Buffer extrapure, 99% is a high-quality zwitterionic buffer widely used in cell culture, molecular biology, and biochemical research. Its exceptional purity and stability make it ideal for maintaining physiological pH in sensitive biological systems. Unlike traditional buffers, HEPES minimizes interference with cellular processes, ensuring reliable results in experiments.
This extrapure grade HEPES buffer is manufactured under stringent quality controls, meeting the highest standards for laboratory use. Whether you’re culturing cells, conducting enzyme assays, or performing protein studies, this buffer provides consistent performance. Researchers and educators trust HEPES for its ability to maintain pH stability even in complex media.
At eqipped, we ensure that every batch of HEPES Buffer extrapure, 99% undergoes rigorous testing to confirm its purity and functionality. This makes it a preferred choice for labs across India, from academic institutions to advanced research facilities.
Need a reliable HEPES buffer for your experiments? Buy HEPES Buffer extrapure, 99% online today for guaranteed quality and fast delivery.
Why HEPES Buffer is Essential for Your Lab
HEPES buffer is a cornerstone in modern biochemistry and cell biology due to its unique properties:
First, it maintains a stable pH range of 6.8 to 8.2, which is critical for cell viability and enzyme activity. Unlike carbonate buffers, HEPES doesn’t produce CO₂, preventing unwanted pH shifts in closed systems. This makes it indispensable for cell culture work, where even minor fluctuations can compromise results.
Second, its low toxicity and minimal interaction with metal ions ensure it doesn’t interfere with cellular metabolism. This is particularly important in sensitive applications like stem cell research or protein crystallization.
Finally, HEPES is compatible with a wide range of biological systems, from mammalian cell lines to bacterial cultures. Its versatility extends to applications like DNA/RNA extraction, where maintaining pH integrity is non-negotiable.
For researchers and students, choosing the right buffer can make or break an experiment. With HEPES Buffer extrapure, 99%, you get consistency, reliability, and peace of mind.
Applications of HEPES Buffer extrapure, 99%
This high-purity HEPES buffer is suitable for:
Cell culture media preparation, where it replaces bicarbonate buffers to avoid CO₂ dependency.
Biochemical assays requiring precise pH control, such as enzyme kinetics studies.
Protein purification and crystallization, where buffer stability is paramount.
Molecular biology techniques like PCR and DNA sequencing, where pH fluctuations can affect amplification.
Electrophysiology experiments, where HEPES is used in patch-clamp solutions to maintain cell health.
Whether you’re a student setting up your first cell culture or a seasoned researcher optimizing a protocol, HEPES Buffer extrapure, 99% delivers the performance you need.
How to Use HEPES Buffer in Your Experiments
Using HEPES buffer effectively requires proper preparation and handling. Here’s a quick guide:
Dissolve the powder in deionized water to achieve the desired concentration (typically 10-50 mM).
Adjust the pH using NaOH or HCl, as HEPES alone doesn’t fully dissolve in water without pH adjustment.
Sterilize the solution by filtration (0.22 µm) if used in cell culture to prevent contamination.
Store the prepared buffer at 4°C for short-term use or aliquot and freeze for long-term storage.
Always check the pH before use, as temperature changes can slightly alter it.
For best results, use fresh buffer for critical experiments, especially in cell culture where degradation over time can affect performance.
Safety and Handling
While HEPES is generally safe, proper lab practices should be followed:
Wear appropriate PPE (gloves, lab coat) when handling the powder to avoid skin or eye irritation.
Avoid inhaling the powder, as it may cause respiratory discomfort.
Dispose of waste according to local regulations, as HEPES is not hazardous but should not be released into the environment.
Store the powder in a cool, dry place, away from direct sunlight and moisture.
Why Researchers Trust eqipped for HEPES Buffer
When you buy HEPES Buffer extrapure, 99% from eqipped, you’re choosing a supplier committed to quality and reliability. Our products are sourced from trusted manufacturers like SRL Chem, ensuring you receive lab-grade materials that meet international standards.
We understand the demands of Indian researchers and educators, which is why we offer competitive pricing, fast shipping, and responsive customer support. Whether you need a small pack for a student project or bulk quantities for a large-scale study, eqipped has you covered.
Our seamless online ordering process makes it easy to get the supplies you need without delays. Plus, our team is always available to assist with technical queries or custom requirements.
FAQs
What is HEPES buffer used for?
HEPES buffer is primarily used in cell culture, biochemical assays, and molecular biology to maintain a stable pH environment. It’s preferred over bicarbonate buffers because it doesn’t rely on CO₂ for pH regulation.
How do I prepare a 1X HEPES buffer solution?
Dissolve HEPES powder in deionized water to reach your desired concentration (e.g., 10-50 mM). Adjust the pH to 7.2-7.4 using NaOH or HCl, then sterilize by filtration if needed.
Is HEPES buffer safe for cell culture?
Yes, HEPES is non-toxic and widely used in cell culture. Its extrapure grade ensures minimal impurities, making it safe for even sensitive cell lines.
Can I use HEPES buffer for PCR?
Absolutely. HEPES is commonly used in PCR buffers to maintain optimal pH for DNA polymerase activity, improving amplification efficiency.
What’s the shelf life of HEPES buffer powder?
When stored properly in a cool, dry place, HEPES buffer powder has a shelf life of 2-3 years. Prepared solutions should be used within a few weeks or stored frozen for longer stability.
