Description
Specifications Table
| Product Name | HEPES Sodium Salt extrapure, 99% |
|---|---|
| Pack Size | 25 Gms, 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Extrapure |
| Application | Cell culture, biochemical assays, pH buffering |
Product Description
HEPES Sodium Salt extrapure, 99% is a premium-grade buffer widely used in laboratories for maintaining stable pH conditions in cell culture media, protein studies, and molecular biology experiments. Its high purity ensures minimal interference in sensitive assays, making it a trusted choice for researchers and educators.
This HEPES buffer powder is particularly effective in biological systems where precise pH control is critical. Unlike traditional buffers, HEPES Sodium Salt provides excellent buffering capacity in the physiological pH range (6.8–8.2), reducing the risk of pH fluctuations during experiments. Whether you’re working with mammalian cell lines, bacterial cultures, or enzyme reactions, this product delivers consistent performance.
At eqipped, we source HEPES Sodium Salt from SRL Chem, a reputable supplier known for stringent quality standards. Each batch undergoes rigorous testing to confirm purity and functionality, ensuring you receive a reliable product every time. For researchers looking to buy HEPES Sodium Salt online, eqipped offers competitive pricing, fast shipping, and hassle-free ordering.
Need HEPES Sodium Salt for your lab? Shop now for guaranteed purity and performance.
Key Applications
HEPES Sodium Salt is versatile and finds use in various laboratory settings:
- Cell culture media preparation to maintain optimal pH for cell growth.
- Biochemical assays requiring stable pH conditions.
- Protein purification and enzyme kinetics studies.
- Electrophysiology experiments where pH stability is crucial.
Why HEPES Sodium Salt?
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic buffer that offers several advantages over traditional buffers like phosphate or Tris:
- Minimal interaction with metal ions, making it ideal for enzymatic reactions.
- Low toxicity to cells, ensuring healthy culture conditions.
- Stable across a wide temperature range, suitable for various protocols.
Handling and Storage
To maximize shelf life and performance, store HEPES Sodium Salt in a cool, dry place away from direct sunlight. Always use clean, dry labware to avoid contamination. For long-term storage, keep the container tightly sealed to prevent moisture absorption.
Looking for a reliable HEPES buffer supplier? Order from eqipped today for lab-ready quality.
Risk Note
HEPES Sodium Salt is generally safe when handled properly. However, avoid inhalation or direct contact with skin and eyes. Use appropriate personal protective equipment (PPE) such as gloves and goggles when handling. In case of accidental exposure, rinse thoroughly with water and seek medical advice if irritation persists.
FAQs
What is HEPES Sodium Salt used for?
HEPES Sodium Salt is primarily used as a buffering agent in cell culture media, biochemical assays, and molecular biology experiments to maintain stable pH conditions.
Is HEPES Sodium Salt suitable for mammalian cell culture?
Yes, HEPES Sodium Salt is widely used in mammalian cell culture due to its low toxicity and effective buffering in the physiological pH range.
How do I prepare a HEPES buffer solution?
Dissolve the required amount of HEPES Sodium Salt in distilled water, adjust the pH to the desired level using HCl or NaOH, and sterilize by filtration if needed.
What is the shelf life of HEPES Sodium Salt?
When stored properly in a cool, dry place, HEPES Sodium Salt has a shelf life of 2–3 years. Always check the expiration date on the packaging.
Can I buy HEPES Sodium Salt in bulk from eqipped?
Yes, eqipped offers HEPES Sodium Salt in multiple pack sizes (25g, 100g, 500g) to suit your lab’s needs. Contact us for bulk inquiries.
