Description
Specifications Table
| Product Name | HEPES Sodium Salt for tissue culture, 99.5% |
|---|---|
| Quantity/Pack Size | 25 Gms, 100 Gms |
| Form | Powder |
| Grade | Tissue Culture Grade |
| Application | Cell culture buffering, biochemical assays, research |
Product Description
What is HEPES Sodium Salt?
HEPES sodium salt is a zwitterionic organic buffering agent widely used in cell culture and biochemical research. Its full name, N-2-Hydroxyethyl piperazine N-2-Ethane Sulphonic Acid Sodium Salt, highlights its role in maintaining stable pH levels in biological systems. Unlike traditional buffers, HEPES sodium salt provides excellent buffering capacity between pH 6.8 and 8.2, making it ideal for delicate cell culture environments.
Why Use HEPES Sodium Salt in Tissue Culture?
Cell culture experiments demand precise pH control to ensure optimal growth conditions. HEPES sodium salt is preferred over bicarbonate buffers because it doesn’t rely on CO₂ levels, reducing variability in experiments. Researchers and students working with mammalian cell lines, primary cultures, or sensitive assays benefit from its stability and non-toxicity.
For reliable cell culture buffering, buy HEPES sodium salt online from eqipped today.
Key Applications of HEPES Sodium Salt
This high-purity HEPES buffer is essential for:
- Maintaining pH stability in cell culture media
- Supporting biochemical assays requiring consistent pH
- Use in protein purification and enzyme studies
- Research involving sensitive cell lines or primary cultures
How to Use HEPES Sodium Salt in Your Lab
To prepare a HEPES buffer solution, dissolve the required amount of HEPES sodium salt in distilled water and adjust the pH using NaOH or HCl. A typical working concentration ranges from 10 to 25 mM, depending on the application. Always filter-sterilize the solution before adding it to cell culture media to prevent contamination.
Safety and Handling
While HEPES sodium salt is generally non-toxic, standard lab safety practices should be followed. Wear appropriate personal protective equipment (PPE) such as gloves and goggles when handling. Store the product in a cool, dry place away from direct sunlight to maintain its stability.
Why Choose eqipped for Your Lab Supplies?
At eqipped, we understand the importance of high-quality reagents in research and education. Our HEPES sodium salt is sourced from trusted manufacturers like SRL Chem, ensuring consistency and reliability in your experiments. Whether you’re a student setting up your first cell culture or a researcher optimizing protocols, eqipped provides the tools you need to succeed.
FAQs
What is the shelf life of HEPES sodium salt?
When stored properly in a sealed container at room temperature, HEPES sodium salt has a shelf life of 2–3 years. Always check the expiration date on the packaging.
Can HEPES sodium salt be autoclaved?
No, HEPES buffers should not be autoclaved as high temperatures can degrade the compound. Instead, filter-sterilize the solution using a 0.22 µm filter.
Is HEPES sodium salt compatible with all cell lines?
HEPES is widely compatible with most mammalian cell lines. However, always verify compatibility with your specific cell type before use.
What is the difference between HEPES and HEPES sodium salt?
HEPES sodium salt is the sodium salt form of HEPES, offering better solubility in water compared to the free acid form. It’s often preferred for cell culture applications.
How do I dispose of HEPES sodium salt waste?
Follow your institution’s guidelines for chemical waste disposal. HEPES sodium salt is generally non-hazardous but should be disposed of responsibly.
