Description
Specifications Table
| Product Name | BioLit® FluroGreen qPCR Master Mix (2X) |
|---|---|
| Quantity/Pack Size | 0.5 ml (20 amps), 1 ml (40 amps), 5 × 1 ml (200 amps) |
| Form | Liquid |
| Grade | Molecular Biology |
| Application | Real-time PCR, gene expression analysis, pathogen detection |
Product Description
When precision matters in your qPCR experiments, the BioLit® FluroGreen qPCR Master Mix (2X) delivers consistent and reliable results. This ready-to-use 2X master mix is formulated with FluroGreen dye, a high-sensitivity fluorescent dye that eliminates the need for SYBR Green I, ensuring accurate quantification of DNA amplification in real-time PCR.
Designed for researchers, students, and educators, this master mix simplifies workflow by combining all essential components—DNA polymerase, dNTPs, MgCl₂, and stabilizers—in a single tube. Whether you’re analyzing gene expression, detecting pathogens, or validating genetic markers, this mix ensures high efficiency and reproducibility.
Available in convenient pack sizes, including 0.5 ml (20 reactions), 1 ml (40 reactions), and 5 × 1 ml (200 reactions), it’s perfect for both small-scale experiments and high-throughput labs. The optimized buffer system minimizes non-specific amplification, while the pre-mixed format reduces pipetting errors, saving you time and effort.
Upgrade your qPCR workflow with BioLit® FluroGreen qPCR Master Mix—order now and experience seamless real-time PCR performance.
Why This Master Mix Stands Out
Unlike traditional SYBR Green-based mixes, FluroGreen offers enhanced stability and reduced inhibition, making it ideal for challenging templates. The 2X concentration ensures flexibility in reaction setup, allowing you to adjust primer and template volumes without compromising performance.
For educators, this master mix is a great teaching tool, helping students understand real-time PCR principles with minimal setup complexity. Researchers will appreciate its compatibility with standard qPCR instruments and its ability to deliver low CV values across replicates.
At eqipped, we ensure that every batch meets stringent quality standards, so you can trust the consistency of your results. Whether you’re in academia or industry, this master mix is a cost-effective solution for high-precision qPCR.
Applications
- Gene expression analysis
- Pathogen detection and quantification
- Genotyping and mutation analysis
- MicroRNA and viral RNA studies
- Educational and training purposes
Storage and Handling
Store the master mix at -20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles to maintain enzyme activity. For best results, aliquot the mix if frequent use is expected.
Risk Note
This product contains components that may be hazardous if mishandled. Always wear appropriate personal protective equipment (PPE) and follow standard laboratory safety protocols. Refer to the Safety Data Sheet (SDS) for detailed handling instructions.
FAQs
What is the difference between FluroGreen and SYBR Green in qPCR?
FluroGreen is a proprietary dye designed for higher stability and lower inhibition compared to SYBR Green, making it more reliable for sensitive qPCR assays.
Can I use this master mix for multiplex qPCR?
Yes, the BioLit® FluroGreen qPCR Master Mix is compatible with multiplex assays, provided primers are optimized for minimal cross-reactivity.
What is the shelf life of this product?
The master mix has a shelf life of 12 months when stored at -20°C. Always check the expiration date on the label.
Is this master mix compatible with all qPCR instruments?
Yes, it works with most real-time PCR systems, including those from Applied Biosystems, Bio-Rad, and Roche.
How do I calculate the volume needed for my experiment?
For a 20 µL reaction, use 10 µL of the 2X master mix, 1 µL of each primer, and adjust the template volume as needed.
