Description
Specifications Table
| Product Name | DH5α Competent Cells for Subcloning |
|---|---|
| Pack Size | 2 × 0.1 mL, 4 × 0.5 mL |
| Form | Liquid (frozen) |
| Grade | Research Grade |
| Application | Subcloning, Bacterial Transformation, Plasmid Propagation |
Product Description
When it comes to reliable subcloning and bacterial transformation, DH5α competent cells are a staple in molecular biology labs. These high-efficiency cells are specifically engineered to maximize transformation success, making them perfect for researchers, students, and educators working on gene cloning, plasmid propagation, or recombinant DNA studies.
DH5α cells are derived from the E. coli DH5α strain, known for its high transformation efficiency and stability. Whether you’re amplifying plasmids, constructing libraries, or performing routine cloning experiments, these competent cells deliver consistent results with minimal background contamination.
At eqipped, we ensure that these cells are handled and stored under optimal conditions to preserve their viability. Each batch is rigorously tested to meet the highest standards, so you can trust them for your critical experiments.
Need high-efficiency competent cells for your next cloning project? Order DH5α competent cells online today and experience seamless bacterial transformation.
FAQs
What is the transformation efficiency of DH5α competent cells?
DH5α competent cells typically achieve a transformation efficiency of 1 × 10^8 to 1 × 10^9 CFU/µg of plasmid DNA, making them highly efficient for most cloning applications.
Can I use DH5α cells for protein expression?
While DH5α cells are excellent for cloning and plasmid propagation, they are not ideal for protein expression due to their lack of certain regulatory elements. For protein expression, consider using BL21 or other expression-specific strains.
How should I store DH5α competent cells?
Store the cells at -80°C in a manual defrost freezer. Avoid storing them in frost-free freezers, as temperature fluctuations can reduce their viability.
What is the shelf life of DH5α competent cells?
When stored properly at -80°C, DH5α competent cells have a shelf life of up to 12 months. Always check the expiration date on the packaging before use.
Are DH5α cells suitable for blue-white screening?
Yes, DH5α cells are commonly used for blue-white screening when working with plasmids containing the lacZ gene. This allows for easy identification of recombinant colonies.
