Description
Specifications Table
| Product Name | Dey Engley Neutralizing Broth w/o BCP |
|---|---|
| Pack Size | 10 × 100 ml |
| Form | Liquid |
| Grade | For Laboratory Use |
| Application | Neutralization of disinfectants, antiseptics, and sanitizers in microbial testing |
Product Description
When working with disinfectants or antiseptics, ensuring accurate microbial testing is crucial. That’s where Dey Engley Neutralizing Broth comes in. This specialized medium is formulated to neutralize the effects of common disinfectants, allowing for precise recovery and enumeration of microorganisms in lab samples.
Unlike standard broths, this version comes without Bromocresol Purple (BCP), making it ideal for applications where color indicators might interfere with results. Whether you’re a student validating lab protocols or a researcher testing sanitizer efficacy, this broth ensures reliable outcomes every time.
Each 100ml bottle is sterile and ready to use, eliminating the need for autoclaving. The 10 × 100ml pack size is perfect for labs with moderate to high testing volumes, offering both convenience and cost-effectiveness.
Need a dependable neutralizing broth for your microbiology experiments? Order Dey Engley Neutralizing Broth online today and get lab-ready supplies delivered to your doorstep.
Why This Broth Matters in Microbiology
Disinfectants and antiseptics are designed to kill microbes, but when testing their efficacy, residual chemicals can skew results. Dey Engley Neutralizing Broth contains specific agents like lecithin, polysorbate 80, and sodium thiosulfate that counteract these chemicals, allowing accurate microbial recovery.
This broth is widely used in:
- Quality control testing of disinfectants
- Environmental monitoring in pharmaceuticals
- Food safety and hygiene validation
- Academic research on antimicrobial resistance
For students and educators, it’s an essential tool for teaching microbial neutralization techniques. Researchers rely on it for reproducible results in antimicrobial studies.
How to Use Dey Engley Neutralizing Broth
Using this broth is straightforward, but proper technique ensures accuracy:
- Prepare your sample by exposing it to the disinfectant or antiseptic under test conditions.
- Transfer the sample into the neutralizing broth to halt antimicrobial activity.
- Incubate at the recommended temperature (usually 35–37°C) for 24–48 hours.
- Subculture onto selective agar plates for enumeration or further testing.
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Storage and Handling
To maintain efficacy, store the broth at 2–8°C in a cool, dark place. Avoid freezing, as this can alter the medium’s properties. Once opened, use within the recommended shelf life to ensure optimal performance.
Always follow standard lab safety protocols when handling microbiological media. While this broth is non-hazardous, proper disposal of used cultures is essential to prevent contamination.
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FAQs
1. What is the shelf life of Dey Engley Neutralizing Broth?
The broth typically has a shelf life of 12–24 months when stored unopened at 2–8°C. Always check the expiration date on the label before use.
2. Can this broth neutralize all types of disinfectants?
It effectively neutralizes quaternary ammonium compounds, phenols, and halogens. For other disinfectants, verify compatibility or use additional neutralizing agents as needed.
3. Is this broth suitable for fungal neutralization?
While primarily designed for bacteria, it can support fungal recovery. For specialized fungal testing, consider supplementary media like Sabouraud Dextrose Broth.
4. How do I know if the neutralization worked?
Compare microbial recovery in neutralized samples against positive controls (untreated samples). Consistent recovery indicates successful neutralization.
5. Can I buy Dey Engley Neutralizing Broth in smaller quantities?
This product is available in a 10 × 100ml pack. For smaller needs, check eqipped’s catalog for alternative sizes or contact support for custom requests.
