Description
Specifications Table
| Product Name | Bile Esculin Agar Base | M340-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Detection of enterococci in clinical and environmental samples |
Product Description
Bile Esculin Agar Base is a specialized microbiological medium designed for the selective isolation and identification of enterococci. This high-quality agar base from Himedia contains bile salts and esculin, which inhibit the growth of Gram-negative bacteria while allowing enterococci to thrive. The hydrolysis of esculin produces a characteristic blackening of the medium, making it easy to identify positive results.
Ideal for laboratories, research institutions, and educational settings, this medium is essential for diagnostic and quality control applications. Whether you’re conducting clinical tests or environmental monitoring, Bile Esculin Agar Base ensures reliable and reproducible results.
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Key Applications
This agar base is widely used in:
- Clinical microbiology for enterococcus detection
- Environmental monitoring of water and food samples
- Research laboratories studying bacterial resistance
- Educational institutions for microbiology training
Why This Product Stands Out
Bile Esculin Agar Base from Himedia is formulated with high-purity ingredients to ensure consistent performance. The inclusion of bile salts and esculin provides a robust selective environment, reducing false positives and improving diagnostic accuracy. Its powder form allows for easy preparation, making it a convenient choice for busy labs.
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Preparation and Usage
To prepare the medium, suspend 500 gm of the powder in 1 liter of distilled water. Heat gently to dissolve completely, then sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C before pouring into sterile Petri dishes. Store prepared plates at 2-8°C for up to 4 weeks.
For best results, inoculate the medium with the test sample and incubate at 35-37°C for 24-48 hours. Observe for blackening of the medium, indicating esculin hydrolysis by enterococci.
Safety and Handling
This product is for laboratory use only. Handle with standard microbiological safety precautions. Wear appropriate personal protective equipment (PPE) when preparing and using the medium. Dispose of used cultures according to local biohazard regulations.
FAQs
What is the shelf life of Bile Esculin Agar Base?
The powder has a shelf life of 2 years when stored in a cool, dry place. Prepared plates should be used within 4 weeks.
Can this medium detect other bacteria besides enterococci?
While primarily designed for enterococci, some other Gram-positive bacteria may also grow. However, the blackening reaction is specific to esculin hydrolysis, typically seen in enterococci.
Is this product suitable for student labs?
Yes, it is commonly used in educational settings for microbiology practicals. Always follow proper lab protocols.
How does bile esculin agar differ from other selective media?
Unlike general-purpose media, this agar specifically targets enterococci due to its bile salts and esculin content, providing clearer differentiation.
Where can I buy bile esculin agar online in India?
eqipped offers authentic Himedia products with fast shipping across India. Order now for hassle-free delivery.
