Description
Specifications Table
| Product Name | Citrate Agar | M728-100G | Himedia |
|---|---|
| Quantity/Pack Size | 100 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Citrate utilization test, microbial identification |
Product Description
Citrate Agar is a specialized microbial medium designed to determine the ability of bacteria to utilize citrate as their sole carbon source. This test is crucial in differentiating enteric bacteria, particularly in clinical and research laboratories. The medium contains sodium citrate as the primary carbon source, along with ammonium salts as the nitrogen source. Bacteria that can utilize citrate produce alkaline byproducts, turning the bromothymol blue indicator from green to blue, confirming a positive result.
This medium is widely used in microbiology labs for identifying organisms like Klebsiella and Enterobacter, which are citrate-positive, while E. coli typically tests negative. The simplicity and reliability of Citrate Agar make it a staple in diagnostic and educational settings.
At eqipped, we ensure that every batch of Citrate Agar meets stringent quality standards, providing consistent and accurate results for your experiments. Whether you’re a student, researcher, or educator, this medium is an essential tool for your microbiology workflow.
How Citrate Agar Works
The principle behind Citrate Agar is straightforward. The medium lacks glucose or other fermentable sugars, forcing bacteria to rely on citrate for growth. If the organism can metabolize citrate, it produces ammonia, raising the pH and changing the indicator color. This visual shift is easy to interpret, making the test both efficient and user-friendly.
For best results, inoculate the slant lightly and incubate at 35-37°C for up to 4 days. A color change from green to blue indicates a positive citrate utilization test.
Applications of Citrate Agar
Citrate Agar is primarily used in:
- Clinical microbiology for bacterial identification
- Research labs studying metabolic pathways
- Educational settings for teaching microbial biochemistry
Its role in differentiating enteric bacteria makes it indispensable in diagnostic workflows, especially when combined with other tests like TSI or Urease.
Why Citrate Agar is Essential for Your Lab
Accuracy and reliability are non-negotiable in microbiology. Citrate Agar provides a clear, unambiguous result, reducing guesswork in bacterial identification. Its long shelf life and ease of preparation further enhance its utility in busy labs.
Need a reliable medium for citrate tests? Buy Citrate Agar online from eqipped today.
Safety and Handling
While Citrate Agar is non-hazardous, standard lab safety protocols should be followed. Wear gloves and a lab coat when handling. Store in a cool, dry place away from direct sunlight.
FAQs
What is the purpose of Citrate Agar?
Citrate Agar tests whether bacteria can use citrate as their sole carbon source, aiding in the identification of enteric bacteria.
How do I interpret Citrate Agar results?
A color change from green to blue indicates a positive result, meaning the organism can utilize citrate.
Can Citrate Agar be used for fungi?
No, this medium is specifically designed for bacterial testing and is not suitable for fungal cultures.
What is the shelf life of Citrate Agar?
When stored properly, unopened Citrate Agar has a shelf life of 2-3 years. Prepared plates should be used within a few days.
Where can I buy Citrate Agar online?
You can purchase high-quality Citrate Agar from eqipped, ensuring fast delivery and competitive pricing.
