Description
Specifications Table
| Product Name | Citrate Agar | M728-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Microbiological testing, citrate utilization studies |
Product Description
Citrate Agar is a specialized microbiological medium designed to identify bacteria capable of utilizing citrate as their sole carbon source. This agar, formulated by Himedia (M728), is widely used in clinical and research laboratories to differentiate enteric bacteria, particularly in the identification of Salmonella and Citrobacter species.
The medium contains sodium citrate as the primary carbon source, along with ammonium salts as the nitrogen source. The inclusion of bromothymol blue as a pH indicator helps visualize citrate utilization—colonies that turn the agar from green to blue indicate a positive result.
Whether you’re a student conducting experiments, a researcher validating bacterial metabolism, or an educator demonstrating microbial biochemistry, this 500 gm pack of Citrate Agar ensures consistent and reliable results. The powdered form allows for easy preparation, and its shelf-stable nature makes it convenient for long-term storage.
Need a reliable citrate agar supplier? eqipped offers high-quality Himedia M728 agar for your lab needs.
Key Applications of Citrate Agar
Citrate Agar is essential in microbiology for several critical applications:
1. Bacterial Identification
Used in the IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) series of tests to distinguish between E. coli (citrate-negative) and Enterobacter or Klebsiella (citrate-positive) species.
2. Clinical Diagnostics
Helps in confirming the presence of citrate-utilizing pathogens in clinical samples, aiding in accurate diagnosis and treatment planning.
3. Educational Labs
An indispensable tool for teaching microbial metabolism and differential staining techniques in universities and colleges.
4. Research Studies
Used in metabolic studies to understand bacterial nutrient utilization patterns under controlled conditions.
How to Prepare Citrate Agar
Preparing Citrate Agar is straightforward, but precision ensures accurate results:
Step 1: Weighing
Measure 24.2 grams of Citrate Agar powder for 1 liter of distilled water. Adjust quantities proportionally for smaller volumes.
Step 2: Dissolving
Add the powder to cold water and mix thoroughly to avoid clumping. Heat gently while stirring until fully dissolved.
Step 3: Sterilization
Autoclave the medium at 121°C for 15 minutes. Avoid overheating to prevent degradation of the pH indicator.
Step 4: Pouring Plates
Cool the medium to 45–50°C and pour into sterile Petri dishes. Allow the agar to solidify before use.
Step 5: Inoculation
Streak or spot-inoculate the test organism and incubate at 35–37°C for 24–48 hours. Observe for color changes.
Interpreting Results
A positive citrate utilization test is indicated by:
- A color shift from green to deep blue in the medium.
- Growth of the organism along the streak line.
A negative result shows no color change, with the medium remaining green.
Storage and Shelf Life
Store the unopened Citrate Agar powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be used within a week if stored at 2–8°C. Avoid freezing, as it may affect the medium’s integrity.
Safety and Handling
While Citrate Agar is non-hazardous, standard lab safety practices should be followed:
- Wear gloves and a lab coat when handling.
- Avoid inhaling the powder to prevent respiratory irritation.
- Dispose of used plates as biohazard waste if contaminated.
Why Citrate Agar Matters in Microbiology
Citrate utilization is a key metabolic trait that helps classify bacteria, especially in the Enterobacteriaceae family. Unlike glucose fermentation tests, citrate agar specifically targets an organism’s ability to use citrate in the absence of other carbon sources. This makes it a valuable tool in:
- Differentiating Salmonella from non-pathogenic strains.
- Confirming the identity of Klebsiella pneumoniae in clinical isolates.
- Teaching students about bacterial enzyme systems.
Looking for citrate agar for microbiology? eqipped provides authentic Himedia M728 agar at competitive prices.
FAQs
1. What is the price of citrate agar 500 gm?
The price of Citrate Agar (M728-500 gm) varies based on bulk orders and promotions. Check eqipped for the latest pricing and discounts.
2. How is citrate agar used in microbiology?
Citrate agar tests an organism’s ability to use citrate as a carbon source. A blue color change indicates a positive result, helping identify bacteria like Klebsiella and Salmonella.
3. Can I buy citrate agar online in India?
Yes, eqipped offers genuine Himedia Citrate Agar (M728) for online purchase with pan-India delivery. Order now for quick dispatch.
4. What is the shelf life of citrate agar powder?
Unopened Citrate Agar powder has a shelf life of 2–3 years when stored in a cool, dry place. Prepared plates should be used within a week.
5. Is citrate agar suitable for student lab experiments?
Absolutely. Its simplicity and clear results make it ideal for educational settings, helping students understand bacterial metabolism and differential testing.
