Description
Specifications Table
| Product Name | Cystine Tellurite Agar Base | M881-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Selective isolation of Corynebacterium diphtheriae |
Product Description
Cystine Tellurite Agar Base (M881) is a specialized microbiological medium designed for the selective isolation and identification of Corynebacterium diphtheriae, the causative agent of diphtheria. This agar base, when supplemented with defibrinated blood, provides an optimal environment for the growth of diphtheria bacilli while inhibiting other bacterial species.
The medium contains cystine, which enhances the growth of C. diphtheriae, and potassium tellurite, which acts as a selective agent by inhibiting the growth of most other bacteria. The beef extract in the formulation supplies essential nutrients, ensuring robust bacterial growth.
This product is widely used in clinical and research laboratories for diagnostic purposes. Whether you’re a student, researcher, or educator, having a reliable supply of Cystine Tellurite Agar Base is crucial for accurate microbiological testing. At eqipped, we ensure that you get high-quality media that meets international standards.
Looking for a trusted supplier of Cystine Tellurite Agar Base? Buy online from eqipped for guaranteed quality and fast delivery.
Key Applications
Cystine Tellurite Agar Base is primarily used for:
- Isolation and identification of Corynebacterium diphtheriae from clinical specimens.
- Differentiation of diphtheria bacilli based on colony morphology and tellurite reduction.
- Research studies involving coryneform bacteria.
Preparation and Usage
To prepare the medium, follow these steps:
- Suspend 500 gm of the powder in 1 liter of distilled water.
- Heat to boiling to dissolve the medium completely.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C and add 5-10% defibrinated blood aseptically.
- Mix well and pour into sterile Petri dishes.
The prepared medium should be stored at 2-8°C and used within the recommended shelf life. Always follow standard microbiological practices when handling and disposing of the medium.
Storage and Handling
Store the powdered medium in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a week for best results. Avoid freezing the prepared medium as it may affect the performance.
Safety Information
Cystine Tellurite Agar Base is generally safe to handle, but standard laboratory precautions should be followed. Wear appropriate personal protective equipment (PPE) such as gloves and lab coats when preparing and using the medium. In case of contact with skin or eyes, rinse immediately with plenty of water.
FAQs
What is Cystine Tellurite Agar Base used for?
It is used for the selective isolation and identification of Corynebacterium diphtheriae from clinical and environmental samples.
How do I prepare Cystine Tellurite Agar Base?
Suspend 500 gm of the powder in 1 liter of distilled water, autoclave, cool to 45-50°C, and add 5-10% defibrinated blood before pouring into plates.
Can I buy Cystine Tellurite Agar Base online?
Yes, you can buy Cystine Tellurite Agar Base online from eqipped, a trusted supplier of microbiology media.
What is the shelf life of Cystine Tellurite Agar Base?
The powdered medium has a shelf life of 2-3 years when stored properly. Prepared plates should be used within a week.
Is Cystine Tellurite Agar Base suitable for student labs?
Yes, it is commonly used in educational settings for teaching microbiology techniques and bacterial identification.
