Description
Specifications Table
| Product Name | Endo HiCynth® Agar |
|---|---|
| Quantity/Pack Size | 100g |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Isolation of coliforms and E. coli |
Product Description
Endo HiCynth® Agar is a specialized microbiological medium designed for the selective isolation and differentiation of coliform bacteria, particularly Escherichia coli. This agar is widely used in water testing, food microbiology, and environmental monitoring due to its ability to inhibit Gram-positive bacteria while promoting the growth of Gram-negative coliforms.
The medium contains lactose as the primary carbohydrate source, which coliforms ferment to produce acid and gas. The presence of basic fuchsin and sodium sulfite in the agar creates a distinctive red coloration around coliform colonies, making identification straightforward. E. coli typically appears as dark red colonies with a metallic sheen, a key visual indicator for microbiologists.
Whether you’re a student setting up a lab experiment or a researcher conducting quality control tests, Endo HiCynth® Agar provides reliable results. Its consistent performance and ease of use make it a staple in microbiology laboratories across India.
Need high-quality agar for your microbiology experiments? Buy Endo HiCynth® Agar online from eqipped for guaranteed purity and fast delivery.
Key Applications
Endo HiCynth® Agar is ideal for:
- Testing water samples for coliform contamination
- Food safety analysis to detect E. coli and other coliforms
- Environmental monitoring in wastewater treatment facilities
- Educational purposes in microbiology labs
Why This Agar Stands Out
Unlike generic agar media, Endo HiCynth® Agar offers superior selectivity due to its optimized formulation. The inclusion of bile salts and sodium lauryl sulfate suppresses the growth of non-coliform bacteria, ensuring accurate results. This makes it a preferred choice for regulatory compliance testing in industries like food and beverage.
For researchers and educators, the clear differentiation of coliform colonies simplifies data interpretation, saving time and reducing errors. The 100g pack size is perfect for small to medium-scale experiments, providing enough medium for multiple tests without excessive waste.
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Usage Guidelines
To prepare Endo HiCynth® Agar, follow these steps:
- Suspend 28.5g of the powder in 1 liter of distilled water.
- Heat to boiling to dissolve the medium completely.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C and pour into sterile Petri dishes.
- Allow the agar to solidify before inoculating samples.
Store the prepared plates at 2-8°C and use within the recommended shelf life for optimal performance.
Safety Information
While Endo HiCynth® Agar is generally safe for laboratory use, always follow standard microbiological practices. Wear appropriate personal protective equipment (PPE) when handling the medium, especially when working with potentially hazardous samples. Dispose of used agar plates according to biohazard waste protocols.
FAQs
What is Endo HiCynth® Agar used for?
Endo HiCynth® Agar is primarily used for the selective isolation and differentiation of coliform bacteria, including E. coli, in water, food, and environmental samples.
How do I interpret the results on Endo HiCynth® Agar?
Coliform colonies typically appear red or pink due to lactose fermentation. E. coli colonies often have a metallic sheen, which helps in their identification.
Can I use Endo HiCynth® Agar for non-coliform bacteria?
No, this medium is specifically formulated to inhibit Gram-positive bacteria and select for coliforms. For other bacteria, consider using a general-purpose agar like Nutrient Agar.
What is the shelf life of Endo HiCynth® Agar?
The powdered medium has a shelf life of 2-3 years when stored in a cool, dry place. Prepared plates should be used within a few weeks for best results.
Where can I buy Endo HiCynth® Agar online in India?
You can purchase authentic Endo HiCynth® Agar (100g) from eqipped, a trusted supplier of laboratory reagents and microbiology media.
