Description
Specifications Table
| Product Name | Fraser Secondary Enrichment Broth Base, Granulated |
|---|---|
| Pack Size | 500 gm |
| Form | Granulated |
| Grade | Microbiology Grade |
| Application | Secondary enrichment for Listeria detection |
Product Description
Fraser Secondary Enrichment Broth Base is a specialized granulated medium designed for the selective enrichment of Listeria species. This high-quality broth is widely used in food microbiology and clinical laboratories to enhance the recovery of Listeria monocytogenes and other Listeria strains from complex samples. The formulation includes selective agents that inhibit the growth of competing microorganisms, ensuring accurate detection.
Whether you’re a researcher, student, or lab technician, this broth provides reliable results for food safety testing, environmental monitoring, and clinical diagnostics. The granulated form ensures easy preparation and consistent performance, making it a trusted choice for microbiologists across India.
Need a reliable medium for Listeria detection? Buy Fraser Secondary Enrichment Broth Base online at eqipped for fast delivery and competitive pricing.
Key Applications
Fraser Secondary Enrichment Broth Base is essential for laboratories involved in:
- Food safety testing for Listeria contamination
- Clinical diagnostics and research
- Environmental monitoring in food processing facilities
- Academic microbiology experiments
Why Use Fraser Broth for Listeria Detection?
Listeria detection requires a two-step enrichment process, and Fraser Secondary Enrichment Broth plays a critical role in the second stage. After primary enrichment in a non-selective medium, samples are transferred to Fraser Broth, where selective agents like lithium chloride and nalidixic acid suppress unwanted bacteria. This ensures that even low levels of Listeria can be detected with high sensitivity.
For students and educators, this broth is an excellent tool for teaching microbiological techniques, as it demonstrates the importance of selective media in pathogen detection. Researchers rely on its consistency for reproducible results in studies involving foodborne pathogens.
Preparation and Usage Guidelines
To prepare Fraser Secondary Enrichment Broth, follow these steps:
- Dissolve 50.5 grams of the granulated medium in 1 liter of distilled water.
- Heat gently while stirring to ensure complete dissolution.
- Autoclave at 121°C for 15 minutes.
- Cool to room temperature before use.
For optimal results, inoculate the broth with a sample from primary enrichment and incubate at 30–37°C for 24–48 hours. Observe for characteristic blackening of the medium, indicating Listeria growth due to aesculin hydrolysis.
Safety and Handling
While Fraser Secondary Enrichment Broth Base is non-hazardous, standard laboratory safety practices should be followed:
- Wear gloves and lab coats when handling.
- Avoid inhalation of powdered medium.
- Dispose of used broth as per biohazard waste protocols.
FAQs
What is Fraser Secondary Enrichment Broth used for?
It is used for the selective enrichment of Listeria species, particularly in food and clinical samples, to enhance detection sensitivity.
How does Fraser Broth differ from primary enrichment media?
Fraser Broth is a secondary enrichment medium with selective agents that inhibit non-Listeria bacteria, unlike primary enrichment media, which are non-selective.
Can I buy Fraser Secondary Enrichment Broth in smaller quantities?
This product is available in a 500g pack. For bulk or custom packaging, contact eqipped for assistance.
What is the shelf life of Fraser Broth Base?
The granulated medium has a shelf life of 2–3 years when stored in a cool, dry place away from direct sunlight.
Is this broth suitable for ISO or FDA-compliant testing?
Yes, Fraser Secondary Enrichment Broth Base meets international standards for Listeria detection in food safety protocols.
