Description
Specifications Table
| Product Name | HiCrome® Aureus Agar Base | M1468 |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Selective isolation and differentiation of Staphylococcus aureus |
Product Description
When it comes to microbiological research, having the right culture media is crucial for accurate results. HiCrome® Aureus Agar Base from Himedia is a specialized medium designed specifically for the selective isolation and differentiation of Staphylococcus aureus. This high-quality agar base is widely used in clinical laboratories, research institutions, and educational settings across India.
The unique formulation of HiCrome® Aureus Agar Base incorporates chromogenic substrates that produce distinctive colony colors, making it easier to identify and differentiate Staphylococcus aureus from other microorganisms. This feature is particularly valuable in diagnostic laboratories where quick and accurate identification is essential for patient care and research.
For students and researchers working on microbiology projects, this agar base provides consistent and reliable results. The 500gm pack size is ideal for laboratories with moderate usage, ensuring you have an adequate supply for multiple experiments without excessive storage requirements.
At eqipped, we understand the importance of quality lab supplies in achieving accurate research outcomes. That’s why we’ve carefully selected HiCrome® Aureus Agar Base from Himedia, a trusted name in microbiology media, to be part of our comprehensive range of laboratory products.
Upgrade your microbiology lab with premium HiCrome® Aureus Agar Base – order your 500gm pack today and experience the difference in your research results.
Key Applications
HiCrome® Aureus Agar Base finds extensive use in various microbiological applications:
Clinical microbiology laboratories use this medium for routine screening of patient samples to detect Staphylococcus aureus infections. The chromogenic properties allow for quick presumptive identification, which is crucial for timely treatment decisions.
In research settings, this agar base is invaluable for studies involving Staphylococcus aureus, including antibiotic resistance research, virulence factor studies, and epidemiological investigations. The selective nature of the medium helps researchers focus on their target organism without interference from other bacteria.
Educational institutions benefit from using HiCrome® Aureus Agar Base in practical microbiology classes. The distinctive colony morphology provides clear visual results that help students understand bacterial identification techniques and the importance of selective media in microbiology.
Food and dairy industries also utilize this medium for quality control testing, ensuring their products are free from Staphylococcus aureus contamination, which is particularly important for food safety and regulatory compliance.
Why This Product Stands Out
Several factors make HiCrome® Aureus Agar Base the preferred choice among microbiologists:
The chromogenic technology provides clear visual differentiation of Staphylococcus aureus colonies, typically appearing as blue to green colonies, while other bacteria are inhibited or appear as different colors. This visual confirmation reduces the need for additional confirmatory tests.
The medium’s selective properties effectively suppress the growth of most Gram-negative bacteria and other Gram-positive organisms, allowing Staphylococcus aureus to grow without competition. This selectivity is crucial for accurate identification and enumeration.
Himedia’s strict quality control ensures batch-to-batch consistency, which is essential for research reproducibility and diagnostic reliability. Each batch undergoes rigorous testing to meet international quality standards.
The powder form allows for flexible preparation – you can make the exact volume needed for your experiments, reducing waste and ensuring fresh media for each use.
Preparation and Usage Guidelines
To achieve optimal results with HiCrome® Aureus Agar Base, follow these preparation guidelines:
Suspend 55 grams of the powder in 1 liter of distilled water. Mix thoroughly to ensure complete dissolution of all components. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
Autoclave at 121°C for 15 minutes. After autoclaving, cool the medium to 45-50°C and pour into sterile Petri dishes. Allow the plates to solidify before use.
For best results, store the prepared plates at 2-8°C and use within a reasonable time frame. The unopened powder should be stored in a cool, dry place away from direct sunlight.
When inoculating, use standard microbiological techniques to avoid contamination. Incubate the plates at 35-37°C for 18-24 hours. Staphylococcus aureus colonies typically appear as blue to green colonies, while other bacteria may be inhibited or appear as different colors.
Safety Considerations
While HiCrome® Aureus Agar Base is generally safe to handle, it’s important to follow standard laboratory safety practices:
Always wear appropriate personal protective equipment including lab coats, gloves, and eye protection when handling microbiological media.
Work in a properly ventilated area, preferably under a biological safety cabinet when handling potentially infectious materials.
Follow your institution’s biosafety guidelines for handling and disposal of microbiological waste.
Store the product away from incompatible materials and food products.
FAQs
What is the shelf life of HiCrome® Aureus Agar Base?
The unopened powder has a shelf life of 3 years when stored properly in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be used within a reasonable time frame, typically within a few weeks when stored at 2-8°C.
Can this medium detect MRSA (Methicillin-resistant Staphylococcus aureus)?
While HiCrome® Aureus Agar Base is excellent for general Staphylococcus aureus detection, it’s not specifically designed for MRSA identification. For MRSA detection, specialized media containing methicillin or oxacillin would be more appropriate. However, this medium can be used as a preliminary screening tool.
What color do Staphylococcus aureus colonies appear on this medium?
On HiCrome® Aureus Agar Base, Staphylococcus aureus colonies typically appear as blue to green colonies. This distinctive coloration is due to the chromogenic substrates in the medium that react with specific enzymes produced by Staphylococcus aureus.
Is this product suitable for student laboratory experiments?
Yes, HiCrome® Aureus Agar Base is excellent for educational purposes. The clear visual results make it ideal for teaching bacterial identification techniques and the principles of selective media. Many universities and colleges across India use this product in their microbiology practical classes.
How does this compare to other Staphylococcus aureus detection media?
HiCrome® Aureus Agar Base offers several advantages over traditional media like Mannitol Salt Agar. The chromogenic properties provide clearer visual identification without the need for additional confirmatory tests in many cases. It also offers better selectivity against other Gram-positive organisms compared to some other media.
