Description
Specifications Table
| Product Name | HiCrome® MacConkey Sorbitol Agar Base |
|---|---|
| Quantity/Pack Size | 100 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | E. coli detection, bacterial isolation |
Product Description
HiCrome® MacConkey Sorbitol Agar Base is a specialized microbiological medium designed for the selective isolation and differentiation of Escherichia coli (E. coli) from clinical and environmental samples. This agar base is widely used in research labs, educational institutions, and diagnostic centers for its reliability in identifying sorbitol-fermenting and non-fermenting strains of E. coli.
The medium contains sorbitol as the primary carbohydrate source, allowing for the differentiation of E. coli O157:H7, which does not ferment sorbitol, from other E. coli strains that do. The inclusion of bile salts and crystal violet inhibits the growth of Gram-positive bacteria, ensuring selective isolation of Gram-negative organisms. The addition of neutral red as a pH indicator helps in visualizing fermentation patterns, making it easier to identify target colonies.
Whether you’re a student working on a microbiology project or a researcher conducting pathogen detection studies, this agar base provides consistent and accurate results. The 100g pack size is ideal for small to medium-scale experiments, ensuring you have enough medium for multiple tests without unnecessary excess.
Looking for a reliable sorbitol macconkey agar supplier? eqipped offers premium-quality HiCrome® MacConkey Sorbitol Agar Base at competitive prices with fast shipping across India.
How to Use HiCrome® MacConkey Sorbitol Agar Base
Preparing and using this agar base is straightforward, but following the correct protocol ensures optimal results:
- Dissolve 50g of the powder in 1 liter of distilled water.
- Heat the solution to boiling to ensure complete dissolution.
- Autoclave at 121°C for 15 minutes to sterilize.
- Cool the medium to 45-50°C before pouring into sterile Petri dishes.
- Allow the agar to solidify before inoculating with the sample.
- Incubate the plates at 35-37°C for 18-24 hours.
- Observe for color changes and colony morphology to identify E. coli.
For best results, always follow standard microbiological practices and maintain sterile conditions during preparation and inoculation.
Applications of MacConkey Sorbitol Agar
This versatile medium is used in various applications, including:
- Detection of E. coli O157:H7 in food and water samples.
- Differentiation of sorbitol-fermenting and non-fermenting E. coli strains.
- Routine microbiological analysis in clinical and research laboratories.
- Educational purposes in microbiology courses for student training.
The ability to distinguish between different E. coli strains makes this agar base invaluable in public health and food safety testing.
Why This Agar Base Stands Out
Not all MacConkey Sorbitol Agar bases are created equal. HiCrome® by Himedia is trusted for its:
- High purity and consistent performance.
- Clear differentiation of E. coli colonies.
- Long shelf life when stored properly.
- Compatibility with standard microbiological protocols.
When you buy macconkey sorbitol agar online from eqipped, you’re choosing a product that meets international quality standards, ensuring reliable results every time.
Storage and Handling
To maintain the integrity of the medium, store the powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a reasonable time frame to prevent dehydration or contamination.
Always wear appropriate personal protective equipment (PPE) when handling microbiological media to minimize exposure risks.
Risk Note
This product is for laboratory use only. Avoid inhalation or direct contact with skin and eyes. In case of accidental exposure, rinse thoroughly with water and seek medical advice if irritation persists. Dispose of used media according to local biohazard waste regulations.
FAQs
What is the difference between MacConkey Agar and MacConkey Sorbitol Agar?
MacConkey Agar uses lactose as the carbohydrate source, while MacConkey Sorbitol Agar uses sorbitol. This difference allows the sorbitol variant to specifically detect E. coli O157:H7, which does not ferment sorbitol, making it a critical tool in pathogen identification.
Can this agar base detect other bacteria besides E. coli?
Yes, while it is optimized for E. coli detection, it can also support the growth of other Gram-negative bacteria like Klebsiella and Enterobacter. However, the selective agents in the medium inhibit most Gram-positive bacteria.
What is the shelf life of HiCrome® MacConkey Sorbitol Agar Base?
The powder has a shelf life of 24 months from the date of manufacture when stored under recommended conditions. Always check the expiration date on the packaging before use.
How do I interpret the results on this agar?
Sorbitol-fermenting E. coli colonies appear pink or red due to the pH indicator, while non-fermenting strains like E. coli O157:H7 appear colorless or pale. This visual distinction helps in quick identification.
Where can I buy macconkey sorbitol agar online in India?
You can purchase HiCrome® MacConkey Sorbitol Agar Base directly from eqipped, a trusted supplier of laboratory reagents and media. We offer competitive pricing, fast shipping, and reliable customer support.
