Description
Specifications Table
| Product Name | HiCrome® MacConkey Sorbitol HiCynth® Agar Base |
|---|---|
| Pack Size | 100 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of E. coli and sorbitol-fermenting bacteria |
Product Description
When it comes to detecting and isolating E. coli in clinical or environmental samples, precision matters. HiCrome® MacConkey Sorbitol HiCynth® Agar Base is a specialized differential medium designed to simplify bacterial identification. This agar base, when supplemented with sorbitol, helps distinguish E. coli from other coliforms, making it indispensable for microbiology labs, research facilities, and educational institutions.
The medium works by leveraging the unique metabolic properties of E. coli. While most coliforms ferment lactose, E. coli O157:H7 does not, allowing for clear differentiation on this agar. The addition of bile salts and crystal violet inhibits Gram-positive bacteria, ensuring selective growth of Gram-negative organisms. The HiCynth® dye enhances colony visibility, making results easier to interpret.
Whether you’re a student learning bacterial identification or a researcher working on pathogen detection, this agar base delivers consistent performance. Its formulation adheres to international microbiological standards, ensuring reliability in your experiments. For those looking to buy MacConkey Sorbitol Agar online, eqipped provides authentic Himedia products with fast delivery across India.
Need a reliable medium for E. coli detection? Order HiCrome MacConkey Sorbitol Agar Base today and ensure accurate results in your lab.
The versatility of this medium extends beyond clinical diagnostics. Environmental microbiologists use it to monitor water quality, while food safety labs rely on it for pathogen screening. Its shelf stability and ease of preparation make it a cost-effective choice for routine testing. When you buy sorbitol MacConkey agar from eqipped, you’re investing in a product trusted by professionals nationwide.
For best results, follow standard microbiological protocols when preparing and inoculating the medium. Store the powder in a cool, dry place and reconstitute as per the manufacturer’s instructions. Always use sterile techniques to prevent contamination. If you’re new to working with selective media, consult your lab manual or reach out to eqipped’s support team for guidance.
FAQs
What is the difference between MacConkey Agar and MacConkey Sorbitol Agar?
MacConkey Agar uses lactose as the fermentable sugar, while MacConkey Sorbitol Agar uses sorbitol. This modification helps identify E. coli O157:H7, which doesn’t ferment sorbitol, making it appear colorless on this medium.
How do I prepare HiCrome MacConkey Sorbitol Agar?
Suspend 52 grams of the powder in 1 liter of distilled water. Heat to boiling while stirring to dissolve completely. Autoclave at 121°C for 15 minutes, then cool to 45-50°C before pouring into sterile plates.
What does a positive result look like on this agar?
Sorbitol-fermenting bacteria produce pink colonies due to the pH indicator. Non-fermenters like E. coli O157:H7 appear colorless. The HiCynth® dye may enhance color differentiation.
Can I use this agar for water testing?
Yes, this medium is suitable for testing water samples for coliforms and E. coli. It’s commonly used in environmental microbiology for water quality assessment.
Where can I buy authentic Himedia MacConkey Sorbitol Agar in India?
You can purchase genuine HiCrome MacConkey Sorbitol HiCynth® Agar Base from eqipped, ensuring you receive a product with full manufacturer support and proper documentation.
