Description
Specifications Table
| Product Name | HiCrome® RajHans® Medium, Modified (Salmonella Agar, Modified) | M1634 |
|---|---|
| Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation and differentiation of Salmonella spp. |
Product Description
When it comes to detecting Salmonella in food, water, or clinical samples, accuracy is non-negotiable. HiCrome® RajHans® Medium, Modified (Salmonella Agar, Modified) is a specialized chromogenic medium designed to simplify and enhance Salmonella identification. This 500gm pack from Himedia ensures consistent performance, making it a trusted choice for labs across India.
Unlike traditional media, this modified formulation incorporates chromogenic substrates that produce distinct colony colors, allowing for easy visual differentiation of Salmonella from other enteric bacteria. The medium suppresses the growth of non-target organisms while promoting the growth of Salmonella, reducing false positives and saving valuable time in diagnostic workflows.
Whether you’re a student conducting microbiology experiments, a researcher validating food safety protocols, or an educator demonstrating bacterial isolation techniques, this medium delivers reliable results. Its user-friendly preparation and long shelf life make it a cost-effective solution for routine and research applications.
Upgrade your lab’s Salmonella detection with HiCrome® RajHans® Medium. Buy online at eqipped for genuine Himedia products with assured quality.
How HiCrome® RajHans® Medium Works
The medium’s unique composition includes selective agents like bile salts and brilliant green that inhibit Gram-positive bacteria and most Gram-negative bacteria except Salmonella. The chromogenic substrate reacts with specific enzymes produced by Salmonella, resulting in characteristic colored colonies (typically pink to red). This visual indicator eliminates the need for additional confirmatory tests in many cases.
For best results, follow standard microbiological practices: 1. Rehydrate the powder as per manufacturer instructions 2. Sterilize by autoclaving at 121°C for 15 minutes 3. Cool to 45-50°C before pouring plates 4. Inoculate samples using standard streaking techniques 5. Incubate at 35-37°C for 18-24 hours
Applications in Research and Education
This versatile medium finds applications across multiple sectors: – Food safety testing in dairy, meat, and processed food industries – Water quality monitoring in environmental labs – Clinical diagnostics for enteric infections – Academic microbiology practicals and research projects – Pharmaceutical quality control testing
Educators appreciate its clear differentiation properties when teaching bacterial identification techniques. The vivid color contrast helps students easily distinguish Salmonella colonies from background flora, reinforcing theoretical concepts with practical observation.
Why Labs Prefer This Formulation
Several factors make HiCrome® RajHans® Medium the preferred choice: – High sensitivity and specificity for Salmonella detection – Reduced need for additional biochemical tests – Long shelf life when stored properly – Cost-effective for high-volume testing – Compatible with automated colony counters
The 500gm pack size offers excellent value for laboratories performing regular Salmonella testing, reducing the frequency of reordering while maintaining freshness of the medium.
Storage and Handling Recommendations
To maintain optimal performance: – Store the unopened powder at 2-8°C in a dry place – Protect from moisture and direct sunlight – Use within the expiration date printed on the package – Once prepared, store plates at 2-8°C and use within 7 days
Always follow your laboratory’s biosafety protocols when handling potential Salmonella samples. Proper disposal of contaminated materials is essential to prevent cross-contamination.
Comparing with Other Salmonella Detection Methods
While traditional methods like XLD or SS agar remain in use, chromogenic media like HiCrome® RajHans® offer significant advantages: – Faster results with clear visual interpretation – Higher specificity reducing false positives – Less subjective interpretation compared to non-chromogenic media – Better recovery of stressed Salmonella cells
For laboratories transitioning to more modern detection methods, this medium provides an excellent balance between traditional reliability and advanced chromogenic technology.
FAQs
What is the shelf life of HiCrome® RajHans® Medium?
The unopened powder has a shelf life of 24 months when stored at 2-8°C. Always check the expiration date on the package before use.
Can this medium detect all Salmonella serotypes?
While highly effective for most common serotypes, some atypical strains may require additional confirmation. The medium is
