Description
Specifications Table
| Product Name | Lactose Egg-Yolk Neomycin Agar Base (LENA) | M21911-500G | Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbial |
| Application | Selective isolation of *E. coli* and coliforms in food, water, and clinical samples |
Product Description
Lactose Egg-Yolk Neomycin Agar Base (LENA) is a specialized microbial medium designed for the selective isolation and differentiation of *Escherichia coli* and other coliform bacteria. This high-quality agar base, manufactured by Himedia (M21911), is widely used in clinical, food, and water microbiology laboratories for accurate detection of enteric pathogens.
The medium contains lactose as a fermentable carbohydrate, egg yolk for lipase activity, and neomycin sulfate as a selective agent to inhibit Gram-positive bacteria. The combination of these components ensures optimal growth of target organisms while suppressing unwanted microbial flora.
Whether you’re a researcher, student, or lab technician, LENA provides reliable results for routine microbiological testing. Its easy-to-prepare powder form allows for convenient media preparation, making it a staple in diagnostic and quality control labs.
Key Features
LENA is formulated to deliver consistent performance with clear colony differentiation. The presence of neomycin ensures suppression of non-target bacteria, while lactose fermentation aids in identifying coliforms through characteristic colony morphology. The egg yolk component enhances the detection of lipolytic activity, further improving diagnostic accuracy.
This medium is particularly useful in food safety testing, where rapid and accurate identification of *E. coli* is critical. It is also employed in clinical microbiology for stool sample analysis and environmental monitoring of water sources.
Applications
LENA is ideal for:
- Isolation of *E. coli* from food and water samples
- Differentiation of coliforms in clinical specimens
- Quality control testing in dairy and food processing industries
- Environmental monitoring for fecal contamination
For best results, follow standard microbiological procedures for media preparation and incubation. Always ensure proper sterilization and aseptic techniques when handling the medium.
Buy Lactose Egg-Yolk Neomycin Agar Base online today for precise microbial analysis.
FAQs
1. What is Lactose Egg-Yolk Neomycin Agar Base used for?
LENA is used for the selective isolation and differentiation of *E. coli* and coliforms in food, water, and clinical samples.
2. How do I prepare Lactose Egg-Yolk Neomycin Agar?
Suspend 50g of the powder in 1 liter of distilled water, heat to boiling, and sterilize by autoclaving at 121?C for 15 minutes. Cool to 45-50?C before pouring into plates.
3. What is the shelf life of LENA?
The powder has a shelf life of 3 years when stored in a cool, dry place. Prepared media should be used within 7 days.
4. Can LENA detect other bacteria besides *E. coli*?
Yes, it can differentiate coliforms based on lactose fermentation and lipase activity, though it is primarily selective for *E. coli*.
5. Is LENA suitable for student lab experiments?
Absolutely! Its straightforward preparation and clear results make it ideal for educational microbiology labs.
