Description
Specifications Table
| Product Name | Listeria Enrichment Medium Base (UVM) | M890A-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective enrichment of Listeria spp. in food, environmental, and clinical samples |
Product Description
When it comes to detecting Listeria monocytogenes and other Listeria species, accuracy and reliability are non-negotiable. The Listeria Enrichment Medium Base (UVM) | M890A-500 gm Himedia is a specialized formulation designed to support the selective enrichment of Listeria spp. from complex samples. Whether you’re working in food safety testing, environmental monitoring, or clinical diagnostics, this medium ensures optimal recovery of target organisms while suppressing competing flora.
Developed by Himedia, a trusted name in microbiological media, this UVM-based medium adheres to international standards for Listeria detection. Its balanced composition includes selective agents that inhibit gram-negative bacteria and other contaminants, allowing Listeria cells to proliferate efficiently. The 500 gm pack size is ideal for labs with moderate to high throughput, providing cost-effective bulk preparation without compromising quality.
For researchers and quality control professionals, consistency is key. This medium delivers reproducible results, making it a staple in ISO, FDA-BAM, and USDA-FSIS compliant protocols. Whether you’re screening dairy products, ready-to-eat foods, or environmental swabs, the Listeria Enrichment Medium Base (UVM) ensures your enrichment step is both sensitive and specific.
Need a reliable medium for Listeria detection? Buy Listeria Enrichment Medium Base (UVM) online from eqipped and streamline your workflow today.
How to Use Listeria Enrichment Medium Base (UVM)
Preparing and using this medium correctly is crucial for accurate results. Follow these steps for optimal performance:
1. Reconstitution: Dissolve 35.5 grams of the medium in 1 liter of distilled water. Heat gently to boiling to ensure complete dissolution, then sterilize by autoclaving at 121°C for 15 minutes.
2. Supplementation: After cooling to 45-50°C, aseptically add selective supplements as per your protocol (e.g., UVM Selective Supplement). Mix well before dispensing.
3. Inoculation: Add the sample (food homogenate, swab, or pre-enrichment broth) to the medium. For food samples, a 1:10 dilution is commonly used.
4. Incubation: Incubate at 30°C for 24-48 hours. Listeria spp. will multiply, while most competing microorganisms are suppressed.
5. Subculturing: After enrichment, streak onto selective agar (e.g., Oxford or PALCAM agar) for isolation and confirmation.
Always refer to your laboratory’s standard operating procedures or regulatory guidelines for specific protocols.
Applications of Listeria Enrichment Medium Base (UVM)
This versatile medium is essential in multiple sectors where Listeria contamination poses a risk:
Food Safety Testing: Used in dairy, meat, seafood, and ready-to-eat food industries to comply with FSIS, FDA, and EU regulations. Detects low levels of Listeria in raw materials and finished products.
Environmental Monitoring: Helps identify Listeria in processing plants, storage facilities, and equipment surfaces, preventing cross-contamination.
Clinical Diagnostics: Employed in hospitals and research labs to isolate Listeria from clinical specimens, aiding in infection control.
Research & Academia: A go-to medium for microbiology students and researchers studying Listeria pathogenesis, biofilm formation, or antimicrobial resistance.
With its broad applicability, the Listeria Enrichment Medium Base (UVM) | M890A is a must-have for any lab focused on pathogen detection.
Why This Medium Stands Out
Not all enrichment media are created equal. Here’s what sets this product apart:
High Selectivity: The UVM formulation effectively inhibits gram-negative bacteria, enterococci, and other competitors, ensuring Listeria cells dominate the enrichment culture.
Consistent Performance: Himedia’s rigorous quality control guarantees batch-to-batch uniformity, reducing variability in your results.
Regulatory Compliance: Meets the requirements of ISO 11290-1, FDA-BAM Chapter 10, and USDA-FSIS protocols for Listeria detection.
Cost-Effective: The 500 gm pack size offers excellent value for labs performing frequent testing, reducing per-test costs.
Ease of Use: Simple reconstitution and supplementation steps minimize preparation time, allowing you to focus on analysis.
For labs that demand precision, this medium delivers results you can trust. Order Listeria Enrichment Medium Base (UVM) online now and experience the difference in your pathogen detection workflow.
Storage and Handling
To maintain the integrity of the medium, follow these storage guidelines:
Unopened Packs: Store in a cool, dry place (15-25°C) away from direct sunlight. The powder is stable for up to 24 months from the date of manufacture.
Prepared Medium: Once reconstituted and supplemented, store at 2-8°C for up to 2 weeks. Avoid repeated freezing and thawing.
Handling Precautions: While this is a non-hazardous product, always wear appropriate PPE (lab coat, gloves, and safety glasses) when handling microbiological media. Dispose of used medium as per your lab’s biohazard waste protocols.
Safety Information
This product is intended for laboratory use only. It is not for human or animal consumption. Although the medium itself is non-toxic, it may support the growth of pathogenic microorganisms after inoculation. Handle with care, and follow standard microbiological practices to prevent contamination.
FAQs
What is the shelf life of Listeria Enrichment Medium Base (UVM)?
The unopened powder has a shelf life of 24 months when stored under recommended conditions. Once prepared, the medium should be used within 2 weeks if stored at 2-8°C.
Can this medium be used for detecting all Listeria species?
Yes, it is designed to enrich all Listeria species, including L. monocytogenes, L. innocua, and L. ivanovii. However, confirmatory tests are required for species-level identification.
Do I need to add supplements to this medium?
Yes, the base medium requires the addition of selective supplements (e.g., UVM Selective Supplement) to achieve full selectivity. These are typically sold separately.
Is this medium suitable for ISO 11290-1 compliance?
Absolutely. This medium is formulated to meet the requirements of ISO 11290-1 for the detection of Listeria monocytogenes in food and environmental samples.
How does this compare to other Listeria enrichment media like Buffered Listeria Enrichment Broth (BLEB)?
While BLEB is used for primary enrichment, UVM medium is often employed as a secondary enrichment step due to its higher selectivity. The choice depends on your specific protocol and regulatory requirements.
