Description
Specifications Table
| Product Name | Lowenstein Jensen Medium Base | M162-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Mycobacterium tuberculosis culture, TB diagnosis |
Product Description
Lowenstein Jensen Medium Base is a specialized nutrient medium designed for the isolation and cultivation of Mycobacterium tuberculosis, the bacterium responsible for tuberculosis (TB). This medium, formulated by Himedia (M162), provides an optimal environment for the slow-growing Mycobacterium species, ensuring reliable results in clinical and research laboratories.
The medium contains essential nutrients like glycerol, potato starch, and mineral salts, which support the growth of Mycobacterium while inhibiting the growth of contaminating bacteria. Its egg-based composition enhances the recovery of TB bacilli, making it a gold standard for TB culture.
Whether you’re a student, researcher, or educator, this medium is ideal for microbiology experiments, diagnostic testing, and academic studies. The 500 gm pack size ensures you have an ample supply for multiple experiments without frequent reordering.
For accurate TB culture results, buy Lowenstein Jensen Medium Base online from eqipped today.
How to Use Lowenstein Jensen Medium Base
1. Preparation: Dissolve the powder in distilled water as per the manufacturer’s instructions. Sterilize by autoclaving at 121°C for 15 minutes.
2. Inoculation: Aseptically inoculate the medium with the clinical sample or bacterial culture.
3. Incubation: Incubate at 35-37°C for 4-8 weeks, as Mycobacterium tuberculosis grows slowly.
4. Observation: Check for characteristic colonies (rough, buff-colored) to confirm TB presence.
Why This Medium is Essential for TB Culture
Lowenstein Jensen Medium Base is preferred in laboratories due to its high selectivity for Mycobacterium species. Unlike general-purpose media, it suppresses the growth of faster-growing contaminants, ensuring accurate identification of TB bacilli. Its egg-based formula also provides a rich nutrient source, improving recovery rates.
For researchers and educators, this medium is invaluable for teaching microbiology techniques and conducting TB-related studies. The 500 gm pack size is cost-effective for institutions and labs with high testing volumes.
Safety and Handling
While Lowenstein Jensen Medium Base is non-hazardous, standard laboratory safety protocols should be followed. Wear gloves and a lab coat when handling. Store in a cool, dry place away from direct sunlight. Always dispose of used media as per biohazard waste guidelines.
FAQs
What is Lowenstein Jensen Medium Base used for?
It is used for the isolation and cultivation of Mycobacterium tuberculosis, the bacterium causing tuberculosis.
How long does it take for TB to grow on this medium?
Mycobacterium tuberculosis typically takes 4-8 weeks to grow on Lowenstein Jensen Medium due to its slow growth rate.
Can this medium be used for other bacteria?
No, it is specifically formulated for Mycobacterium species and inhibits most other bacteria.
What is the shelf life of Lowenstein Jensen Medium Base?
The powder has a shelf life of 2 years when stored properly in a cool, dry place.
Where can I buy Lowenstein Jensen Medium Base online?
You can buy it from eqipped, a trusted supplier of laboratory reagents and media.
