Description
Specifications Table
| Product Name | MacConkey Agar without CV, NaCl with 0.5% Sodium Taurocholate |
|---|---|
| Pack Size | 100 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Gram-negative bacteria, differential lactose fermentation |
Product Description
MacConkey Agar without CV, NaCl with 0.5% Sodium Taurocholate is a specialized microbiological medium designed for the selective isolation and differentiation of Gram-negative bacteria. This formulation excludes Crystal Violet (CV) and includes Sodium Taurocholate, enhancing its ability to inhibit Gram-positive bacteria while promoting the growth of enteric pathogens.
Ideal for clinical, research, and educational laboratories, this medium is particularly useful in identifying lactose-fermenting and non-lactose-fermenting bacteria. The addition of Sodium Taurocholate improves the recovery of bile-tolerant organisms, making it a reliable choice for fecal and environmental samples.
When preparing MacConkey Agar, ensure proper sterilization and cooling to 45-50°C before pouring into Petri dishes. The medium should appear light straw-colored and slightly opaque. Incubate inoculated plates at 35-37°C for 18-24 hours for optimal results.
Need a reliable medium for bacterial isolation? Shop MacConkey Agar without CV online at eqipped for guaranteed quality.
Key Features
This MacConkey Agar variant offers several advantages for microbiological applications:
Selective properties due to the absence of Crystal Violet and inclusion of Sodium Taurocholate.
Differential capabilities based on lactose fermentation, with lactose fermenters producing pink colonies.
Suitable for both routine diagnostics and research applications.
Easy to prepare and store, with a long shelf life when kept in cool, dry conditions.
Applications
MacConkey Agar without CV, NaCl with 0.5% Sodium Taurocholate is widely used in:
Clinical microbiology for isolating enteric pathogens from stool samples.
Environmental monitoring to detect coliforms in water and food.
Educational laboratories for teaching bacterial identification techniques.
Research settings for studying Gram-negative bacterial behavior.
Storage and Handling
Store the powder in a tightly sealed container at 2-8°C.
Prepared plates should be stored at 2-8°C and used within 4 weeks.
Avoid exposure to moisture and direct sunlight.
Safety Information
This product is for laboratory use only. Handle with standard microbiological safety practices.
Wear appropriate personal protective equipment (PPE) when preparing and handling.
Dispose of used media according to local biohazard waste regulations.
Why This Product Stands Out
eqipped brings you Himedia’s MacConkey Agar without CV, a trusted formulation used in laboratories worldwide. Our commitment to quality ensures you receive a product that meets international standards for microbiological media. Whether you’re a student, researcher, or lab technician, this medium provides consistent results for your bacterial isolation needs.
FAQs
What is MacConkey Agar without CV, NaCl with 0.5% Sodium Taurocholate used for?
MacConkey Agar without Crystal Violet (CV), NaCl, containing 0.5% Sodium Taurocholate is a selective and differential culture medium used for the isolation and differentiation of Gram-negative enteric bacteria based on their lactose fermentation characteristics in microbiology laboratories.
Is HiMedia M082 suitable for microbiological research and quality control?
Yes, HiMedia M082 is suitable for microbiology laboratories, pharmaceutical quality control, food and beverage testing, water testing, environmental monitoring, research laboratories, and educational institutions performing routine microbial analysis.
How should MacConkey Agar M082 be prepared and stored?
Prepare the medium according to the manufacturer’s instructions and sterilize as recommended. Store the dehydrated medium in a tightly closed container in a cool, dry place, while prepared plates should be refrigerated and used within the recommended storage period.
What safety precautions should I follow while handling this culture medium?
Wear appropriate laboratory PPE, including gloves, a lab coat, and safety goggles. Prepare and use the medium following standard microbiological laboratory practices to minimize contamination and ensure reliable culture results.
Can I buy HiMedia MacConkey Agar M082 online from eqipped?
Yes, eqipped supplies genuine HiMedia microbiology culture media with secure packaging, reliable delivery across India, and bulk purchasing options for research laboratories, pharmaceutical companies, educational institutions, and industrial quality control laboratories.
