Description
Specifications Table
| Product Name | Polymyxin Pyruvate Egg Yolk Mannitol Bromothymol Blue Agar Base (PEMBA) |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Selective isolation and identification of Bacillus cereus |
Product Description
Polymyxin Pyruvate Egg Yolk Mannitol Bromothymol Blue Agar Base (PEMBA) is a specialized microbiological medium designed for the selective isolation and identification of Bacillus cereus. This agar base is widely used in food, dairy, and clinical laboratories to detect the presence of B. cereus, a common foodborne pathogen.
The medium contains polymyxin to inhibit gram-negative bacteria, egg yolk to enhance lecithinase activity, mannitol as a fermentable carbohydrate, and bromothymol blue as a pH indicator. The combination of these components ensures accurate and reliable results, making PEMBA Agar Base an essential tool for microbiologists and researchers.
When preparing PEMBA Agar, the medium is typically supplemented with additional reagents as per standard protocols. The resulting agar plates allow for the differentiation of B. cereus colonies based on their characteristic appearance, including lecithinase activity and mannitol fermentation.
For researchers and students working in microbiology, PEMBA Agar Base provides a cost-effective and efficient solution for Bacillus cereus detection. Whether you’re conducting food safety testing or academic research, this medium delivers consistent performance.
Looking for a reliable source to buy Polymyxin Pyruvate Agar Base online? eqipped offers high-quality microbiology media at competitive prices.
FAQs
What is the shelf life of PEMBA Agar Base?
The powdered medium has a shelf life of 2-3 years when stored properly. Prepared agar plates should be used within 1-2 weeks for best results.
Can PEMBA Agar Base be used for other bacteria besides Bacillus cereus?
While primarily designed for B. cereus, the medium may support the growth of other Bacillus species. However, its selective components make it most effective for cereus detection.
Is PEMBA Agar Base suitable for student laboratory experiments?
Yes, this medium is commonly used in educational settings for microbiology practicals, especially those focusing on foodborne pathogens.
How does bromothymol blue help in identification?
Bromothymol blue acts as a pH indicator. Bacillus cereus ferments mannitol, producing acid that turns the medium yellow, while lecithinase activity creates a precipitate halo.
Where can I buy Polymyxin Pyruvate Agar Base online in India?
You can purchase high-quality PEMBA Agar Base from eqipped, a trusted supplier of laboratory media and reagents for students, researchers, and educators.
