Description
Specifications Table
| Product Name | R2A Broth | M1687-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Environmental microbiology, water testing, biofilm studies |
Product Description
R2A Broth is a specialized microbiological medium designed to support the growth of bacteria from nutrient-poor environments. Unlike standard nutrient-rich media, R2A Broth contains lower concentrations of peptones and sugars, making it ideal for isolating slow-growing or environmentally stressed microorganisms. This medium is widely used in water quality testing, soil microbiology, and biofilm research.
Whether you’re a student conducting lab experiments or a researcher studying microbial diversity, R2A Broth provides a reliable foundation for your work. Its balanced formulation ensures consistent results, making it a staple in environmental microbiology labs across India.
Looking for R2A Broth suppliers? eqipped offers authentic Himedia products with fast shipping.
Why R2A Broth is Essential for Your Lab
Many standard media fail to recover bacteria from oligotrophic environments like drinking water or soil. R2A Broth bridges this gap by providing just enough nutrients to sustain growth without overwhelming delicate organisms. This makes it particularly useful for:
- Isolating heterotrophic bacteria from water samples
- Studying microbial communities in low-nutrient habitats
- Testing biofilm formation in industrial settings
For researchers working with environmental samples, R2A Broth often yields higher colony counts than richer media like Nutrient Agar. Its versatility extends to both qualitative and quantitative microbiological analyses.
How to Prepare R2A Broth
Preparing R2A Broth is straightforward. Suspend 18.2 grams of the powder in 1 liter of distilled water. Heat gently while stirring until fully dissolved. Sterilize by autoclaving at 121°C for 15 minutes. For agar plates, add 15 grams of agar before autoclaving.
Always follow standard aseptic techniques when handling the medium. Store the prepared broth at 2-8°C and use within the recommended shelf life for optimal performance.
Applications in Research and Education
From undergraduate labs to advanced research facilities, R2A Broth serves multiple purposes:
In academic settings, it helps students understand microbial growth requirements and environmental adaptations. Researchers use it to study microbial ecology, particularly in aquatic systems where nutrient levels are naturally low.
Industrial applications include monitoring water quality in pharmaceutical manufacturing and assessing microbial contamination in food processing environments. The medium’s sensitivity makes it valuable for detecting low levels of bacterial contamination.
Quality Assurance
This Himedia R2A Broth (M1687) undergoes rigorous quality control to ensure batch-to-batch consistency. Each 500gm pack is manufactured under strict conditions to meet international microbiology standards.
When you buy R2A Broth online from eqipped, you receive a product that’s been tested for performance and reliability. Our supply chain maintains proper storage conditions to preserve the medium’s efficacy.
Storage and Handling
Store the unopened powder at room temperature in a dry place. After opening, keep the container tightly sealed to prevent moisture absorption. Prepared broth should be refrigerated and used within a month for best results.
Always check for signs of contamination before use. Cloudiness or unusual odors in prepared media indicate potential issues.
FAQs
What is R2A Broth used for?
R2A Broth is primarily used for culturing bacteria from nutrient-poor environments like water and soil. It’s particularly effective for isolating heterotrophic bacteria that might not grow well on richer media.
How does R2A Broth differ from Nutrient Broth?
R2A Broth contains significantly lower concentrations of peptones and sugars compared to Nutrient Broth. This makes it better suited for environmental samples where standard media might inhibit the growth of certain bacteria.
Can I use R2A Broth for water testing?
Yes, R2A Broth is commonly used in water microbiology for heterotrophic plate counts. It’s often preferred over other media because it recovers a broader range of bacteria from water samples.
What’s the shelf life of R2A Broth powder?
The unopened powder typically has a shelf life of 2-3 years when stored properly. Always check the manufacturer’s expiration date on the packaging.
Where can I buy authentic R2A Broth in India?
You can buy R2A Broth online from eqipped, where we offer genuine Himedia products with reliable delivery across India. Our platform ensures you receive quality lab supplies for your research needs.
