Description
Specifications Table
| Product Name | Sabouraud Dextrose Broth | MH033-100G | Himedia |
|---|---|
| Pack Size | 100 gm |
| Form | Powder |
| Grade | Microbiological |
| Application | Fungal culture, isolation, and identification |
Product Description
Sabouraud Dextrose Broth is a widely used microbiological medium designed for the cultivation and isolation of fungi. This broth formulation by Himedia supports the growth of a broad spectrum of fungal species, making it an essential tool in clinical, research, and industrial laboratories. The medium contains peptone and dextrose, which provide the necessary nutrients for fungal metabolism, while the acidic pH (around 5.6) inhibits bacterial growth, allowing selective fungal recovery.
Ideal for Mycological Studies
Whether you are a student conducting academic research or a professional working in a diagnostic lab, Sabouraud Dextrose Broth offers consistent performance. Its ability to promote luxuriant fungal growth makes it particularly useful for identifying pathogenic fungi such as Candida, Aspergillus, and Cryptococcus. The powder form allows for easy preparation, ensuring fresh batches whenever needed.
This medium is also valuable in environmental monitoring, where fungal contamination needs to be assessed in air, water, or surfaces. Researchers studying antifungal agents or microbial interactions will find this broth indispensable for maintaining pure fungal cultures.
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Applications in Medical and Industrial Labs
In clinical microbiology, Sabouraud Dextrose Broth is often used as a preliminary enrichment medium before plating on Sabouraud Dextrose Agar. This two-step process enhances the detection of fungi in samples where their numbers may be low. The broth’s versatility extends to industries like food and beverage, pharmaceuticals, and cosmetics, where fungal quality control is critical.
For educators, this medium serves as an excellent teaching aid. It allows students to observe fungal morphology and growth patterns firsthand, reinforcing theoretical knowledge with practical experiments. The ease of use and reliability of Himedia’s formulation make it a preferred choice among institutions.
Why This Medium Stands Out
Himedia’s Sabouraud Dextrose Broth is manufactured under stringent quality control measures, ensuring batch-to-batch consistency. The dehydrated powder has a long shelf life when stored properly, making it cost-effective for laboratories with varying usage rates. Its balanced composition minimizes the risk of overgrowth by fastidious organisms, providing clearer results.
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Preparation and Storage Guidelines
To prepare the medium, dissolve the powder in distilled water as per the manufacturer’s instructions, sterilize by autoclaving, and adjust the pH if necessary. Once prepared, the broth should be stored in a cool, dry place away from direct sunlight. For best results, use the medium within its expiry date and follow standard aseptic techniques during handling.
Safety Considerations
While Sabouraud Dextrose Broth is not classified as a high-risk chemical, standard laboratory safety practices should be followed. Wear appropriate personal protective equipment (PPE) such as gloves and lab coats when handling the powder and prepared medium. Avoid inhalation of the dry powder, and ensure proper ventilation in the workspace.
FAQs
What is Sabouraud Dextrose Broth used for?
Sabouraud Dextrose Broth is primarily used for the cultivation and isolation of fungi, including pathogenic species like Candida and Aspergillus. It is commonly employed in clinical, research, and industrial laboratories for mycological studies.
How do I prepare Sabouraud Dextrose Broth from the powder?
Dissolve the required amount of powder in distilled water, heat if necessary to dissolve completely, and sterilize by autoclaving at 121°C for 15 minutes. Allow the medium to cool before use.
Can Sabouraud Dextrose Broth be used for bacterial culture?
No, the acidic pH of Sabouraud Dextrose Broth is optimized to inhibit bacterial growth and selectively promote fungal growth. For bacterial culture, other media like Nutrient Broth or Luria Bertani Broth are recommended.
Where can I buy Sabouraud Dextrose Broth 100g online in India?
You can buy Sabouraud Dextrose Broth 100g online from eqipped, a trusted supplier of laboratory media and reagents. We offer genuine Himedia products with fast shipping across India.
What is the shelf life of Sabouraud Dextrose Broth powder?
When stored in a cool, dry place away from direct sunlight, the unopened powder has a shelf life of typically 3 to 5 years. Always check the manufacturer’s expiry date on the packaging.
