Description
Specifications Table
| Product Name | Selenite Broth Base w/o Biselenite | M970-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Selective enrichment of Salmonella and Shigella in food and water samples |
Product Description
High-Quality Selenite Broth Base for Reliable Microbiological Testing
When it comes to isolating and identifying pathogens like Salmonella and Shigella in food, water, or clinical samples, the choice of enrichment medium matters. The Selenite Broth Base w/o Biselenite from Himedia is a trusted formulation designed to inhibit the growth of non-target bacteria while promoting the proliferation of enteric pathogens. This makes it an essential medium for microbiology labs, research institutions, and quality control departments.
Whether you are a student working on a microbiology project, a researcher conducting pathogen studies, or a lab technician performing routine testing, this broth base ensures accurate and consistent results. The absence of biselenite allows for greater flexibility in customizing the medium to suit specific experimental needs.
Buy Selenite Broth Base online from eqipped and ensure your lab is equipped with the best reagents for precise microbiological analysis.
How Does Selenite Broth Base Work?
Selenite Broth Base contains sodium selenite, which acts as a selective agent. The high concentration of selenite inhibits the growth of many Gram-positive and some Gram-negative bacteria, while allowing Salmonella and Shigella to multiply. This selective pressure is crucial for isolating target pathogens from mixed microbial populations.
The medium also includes peptones and other nutrients that support the growth of the desired organisms. When prepared according to standard protocols, it provides an optimal environment for the enrichment of enteric pathogens before plating on selective agars like XLD or MacConkey.
For labs that require a more tailored approach, the version without biselenite allows researchers to add their own supplements or modify the formulation as needed.
Applications of Selenite Broth Base in Microbiology
The Selenite Broth Base w/o Biselenite is widely used in various microbiological applications, including:
Food Safety Testing: Detecting Salmonella contamination in dairy products, meat, poultry, and processed foods. Water Quality Analysis: Identifying pathogenic bacteria in municipal and industrial water supplies. Clinical Diagnostics: Isolating Shigella and Salmonella from stool samples in medical laboratories. Research Studies: Supporting academic and industrial research on enteric pathogens and their behavior in different environments.
Its versatility and reliability make it a staple in microbiology labs across India. Whether you are testing food samples for compliance or conducting epidemiological studies, this broth base delivers consistent performance.
Looking for a reliable selenite broth supplier in India? Shop now at eqipped for authentic Himedia products.
Preparation and Usage Guidelines
To prepare the Selenite Broth, dissolve the powder in distilled water as per the manufacturer’s instructions. Sterilize the medium by autoclaving at 121°C for 15 minutes. Once cooled, the broth is ready for inoculation with the sample.
For best results, follow standard microbiological practices when handling and incubating the medium. Incubate the inoculated broth at 35-37°C for 18-24 hours before subculturing onto selective agars. Always ensure proper aseptic techniques to avoid contamination.
Note: This product is for laboratory use only. Not for human or animal consumption. Handle with care, especially when working with pathogenic organisms.
Why Microbiologists Trust Himedia’s Selenite Broth Base
Himedia is a globally recognized brand in microbiology media, known for its stringent quality control and consistency. The Selenite Broth Base w/o Biselenite (M970-500 gm) is no exception. It is manufactured under strict GMP guidelines, ensuring batch-to-batch uniformity and reliability.
Labs in India and worldwide rely on Himedia products for their accuracy and performance. When you buy from eqipped, you are getting a product that meets international standards, backed by a trusted supplier.
Order Selenite Broth Base today and experience the difference in your microbiology workflow.
Storage and Shelf Life
Store the powdered medium in a cool, dry place, away from direct sunlight. Once prepared, the broth should be used within a short period or stored under refrigeration if not used immediately. Always check the expiration date on the package and avoid using expired media, as this can affect results.
For long-term storage, keep the container tightly sealed to prevent moisture absorption, which can degrade the product.
Risk Note
Caution: This product is for laboratory and research use only. Not for human or animal consumption. Sodium selenite is toxic in high concentrations. Handle with care and dispose of according to local regulations.
FAQs
What is Selenite Broth Base used for?
Selenite Broth Base is a selective enrichment medium used to isolate Salmonella and Shigella from food, water, and clinical samples by inhibiting competing bacteria.
How do you prepare Selenite Broth from the base?
Dissolve the powder in distilled water, autoclave at 121°C for 15 minutes, and cool before use. Follow the manufacturer’s instructions for exact concentrations.
Can I buy Selenite Broth Base online in India?
Yes, you can buy Selenite Broth Base online from eqipped, a trusted supplier of Himedia lab products in India with fast delivery.
What is the difference between Selenite Broth with and without Biselenite?
The version without biselenite allows researchers to customize the medium by adding their own supplements, offering greater flexibility for specific applications.
Is Selenite Broth Base safe for handling?
While the powder itself is non-hazardous, it is intended for laboratory use only. Always follow standard safety protocols, including wearing gloves and a lab coat when handling.
