Description
Specifications Table
| Product Name | Quantity/Pack Size | Form | Grade | Application |
|---|---|---|---|---|
| Shigella Broth Base | M1326 | 500 gm | Powder | Microbiological Grade | Selective enrichment for Shigella isolation |
Product Description
Shigella Broth Base is a specialized microbiological medium designed to support the growth and isolation of Shigella species from clinical and environmental samples. This formulation provides an optimal environment for the enrichment of Shigella while suppressing the growth of competing microorganisms, making it an essential tool for diagnostic and research laboratories.
Used in conjunction with selective agents, Shigella Broth Base enhances the recovery of Shigella from mixed cultures, improving detection rates in stool samples and other clinical specimens. Its balanced composition ensures consistent performance, making it a reliable choice for routine microbiology workflows.
Whether you are a student conducting experimental work or a researcher analyzing bacterial strains, this medium delivers the precision and reliability needed for accurate results. Shop Shigella Broth Base online at eqipped for lab-grade quality.
Manufactured by Himedia, a trusted name in microbiological media, this product adheres to stringent quality standards, ensuring batch-to-batch consistency. Its powder form allows for easy preparation, and the 500 gm pack size is ideal for laboratories with moderate to high usage.
For best results, follow the manufacturer’s instructions for reconstitution and inoculation. Store the powder in a cool, dry place, away from direct sunlight, to maintain its efficacy. Always use sterile techniques to prevent contamination.
Applications of Shigella Broth Base
Shigella Broth Base is widely used in clinical microbiology for the isolation and identification of Shigella species, which are common causes of bacterial dysentery. It is also employed in food and water testing to detect the presence of pathogenic bacteria.
Researchers studying enteric pathogens rely on this medium for its ability to selectively enhance Shigella growth. Educational institutions use it in practical microbiology courses to demonstrate selective media principles.
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Preparation and Usage Guidelines
To prepare Shigella Broth Base, dissolve the powder in distilled water as per the recommended concentration. Autoclave the medium to sterilize it before use. Once cooled, it can be supplemented with selective agents like novobiocin or bile salts to enhance selectivity.
Inoculate the broth with the sample and incubate at 35–37°C for 18–24 hours. After incubation, streak the broth onto a selective agar medium, such as MacConkey Agar or XLD Agar, for further isolation and identification.
Proper handling and disposal of microbiological media are essential to prevent environmental contamination. Always follow laboratory safety protocols when working with potentially hazardous microorganisms.
FAQs
What is Shigella Broth Base used for?
Shigella Broth Base is a selective enrichment medium used to isolate Shigella species from clinical, food, and water samples. It helps in the preliminary identification of these pathogens before further testing.
How do I prepare Shigella Broth Base?
Dissolve the powder in distilled water according to the manufacturer’s instructions, typically at a concentration of 20–30 gm per liter. Autoclave the solution at 121°C for 15 minutes to sterilize it before use.
Can Shigella Broth Base be used without selective supplements?
While Shigella Broth Base can be used as is, adding selective supplements like novobiocin or bile salts improves its ability to inhibit non-target bacteria, enhancing Shigella recovery.
Where can I buy Shigella Broth Base online in India?
You can buy Shigella Broth Base online from eqipped, a trusted supplier of high-quality lab consumables. We offer competitive pricing and fast delivery across India.
What is the shelf life of Shigella Broth Base?
When stored in a cool, dry place and kept in its original packaging, Shigella Broth Base typically has a shelf life of 2–3 years. Always check the expiration date on the label before use.
