Triple Sugar Iron Agar (In accordance with IP 1996) | MM021-100G | Himedia

Description

Specifications Table

Product Name – Triple Sugar Iron Agar (In accordance with IP 1996)

Quantity/Pack Size – 100g

Form – Powder

Grade – Analytical

Application – Differential bacterial identification

Product Overview

Triple Sugar Iron Agar (TSI) is a specialized medium designed for the differentiation and identification of enteric bacteria based on their ability to ferment glucose, lactose, and sucrose, as well as their production of hydrogen sulfide. This agar is formulated in accordance with the Indian Pharmacopoeia (IP) 1996 standards, ensuring high quality and reliability for laboratory use. The medium contains three sugars (glucose, lactose, and sucrose) and an indicator (phenol red) that changes color based on the pH changes resulting from sugar fermentation. The presence of ferrous sulfate allows for the detection of hydrogen sulfide production, which is indicated by the formation of black precipitates. This agar is particularly useful in clinical microbiology for the identification of pathogens such as Salmonella, Shigella, and Escherichia coli. Its formulation supports accurate and reproducible results, making it an essential tool for students, researchers, and educators in the field of microbiology.

FAQs

1. What is Triple Sugar Iron Agar used for?

Triple Sugar Iron Agar is used for the differential identification of enteric bacteria based on their ability to ferment glucose, lactose, and sucrose, as well as their production of hydrogen sulfide.

2. Is this product compliant with IP 1996 standards?

Yes, this Triple Sugar Iron Agar is formulated in accordance with the Indian Pharmacopoeia (IP) 1996 standards, ensuring high quality and reliability for laboratory use.

3. What are the key components of Triple Sugar Iron Agar?

The key components include glucose, lactose, sucrose, phenol red indicator, and ferrous sulfate for hydrogen sulfide detection.

4. Can this agar be used for clinical microbiology?

Yes, Triple Sugar Iron Agar is particularly useful in clinical microbiology for the identification of pathogens such as Salmonella, Shigella, and Escherichia coli.

5. How does the indicator in TSI Agar work?

The phenol red indicator in TSI Agar changes color based on the pH changes resulting from sugar fermentation, helping to identify the type of bacteria present.