Description
Specifications Table
| Product Name | Tryptone Lactose Iron HiVeg® Agar | MV321-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Differentiation of lactose fermenters and H2S producers |
Product Description
Tryptone Lactose Iron HiVeg® Agar is a specialized microbiological medium designed for the detection and differentiation of microorganisms based on their ability to ferment lactose and produce hydrogen sulfide. This medium is widely used in clinical, research, and educational laboratories to identify enteric bacteria, particularly those involved in gastrointestinal infections.
The formulation includes tryptone, which provides essential nutrients for bacterial growth, lactose as the fermentable carbohydrate, and iron salts to detect hydrogen sulfide production. The HiVeg® variant ensures high purity and consistency, making it a reliable choice for accurate microbial analysis.
Whether you’re a student conducting microbiology experiments or a researcher studying bacterial metabolism, this agar offers precise results for your tests. Its versatility makes it a staple in labs across India, where quality and affordability are key considerations.
Need a dependable medium for your microbiology experiments? Buy Tryptone Lactose Iron HiVeg® Agar online today and ensure accurate results every time.
Key Applications
This medium is commonly used for:
- Differentiating lactose-fermenting bacteria from non-fermenters.
- Detecting hydrogen sulfide production in bacterial cultures.
- Isolating and identifying enteric pathogens in clinical samples.
- Educational purposes in microbiology labs.
Why This Medium Stands Out
Unlike conventional media, Tryptone Lactose Iron HiVeg® Agar offers enhanced clarity and sensitivity, ensuring that even subtle biochemical reactions are easily observable. The inclusion of iron salts allows for the formation of black precipitates when hydrogen sulfide is produced, providing a clear visual indicator for identification.
For labs in India, where cost-effectiveness and reliability are crucial, this medium from HiMedia delivers consistent performance without compromising on quality. Whether you’re setting up a new lab or restocking your supplies, eqipped ensures you get the best products at competitive prices.
Usage Guidelines
To prepare the medium, suspend 50 grams of the powder in 1 liter of distilled water. Heat gently to dissolve completely, then sterilize by autoclaving at 121°C for 15 minutes. Pour into sterile Petri dishes and allow to solidify before inoculation.
For optimal results, incubate inoculated plates at 35-37°C for 18-24 hours. Observe for color changes and blackening due to hydrogen sulfide production.
Safety and Storage
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a reasonable timeframe to maintain efficacy.
FAQs
What is Tryptone Lactose Iron HiVeg® Agar used for?
It is used to differentiate bacteria based on lactose fermentation and hydrogen sulfide production, commonly in microbiology labs.
How do I prepare this medium?
Dissolve 50 grams of the powder in 1 liter of distilled water, sterilize by autoclaving, and pour into Petri dishes.
Can this medium detect E. coli?
Yes, it helps identify E. coli and other lactose-fermenting enteric bacteria.
What is the shelf life of this product?
The powder has a long shelf life when stored properly, typically 2-3 years from the date of manufacture.
Where can I buy Tryptone Lactose Iron HiVeg® Agar online?
You can purchase it from eqipped, a trusted supplier of lab reagents in India.
