Description
Specifications Table
| Product Name | Dulbecco’s Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) |
|---|---|
| Quantity/Pack Size | 20 × 500 ml |
| Form | Liquid |
| Grade | Cell Culture Tested |
| Application | Cell Culture |
Product Description
Dulbecco’s Modified Eagle Medium / Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) is a versatile and widely used medium for cell culture applications. This 1:1 mixture of DMEM and F-12 provides a balanced nutrient environment that supports the growth of a variety of cell types, including primary cells, stem cells, and transformed cell lines.
Why Choose DMEM/F12 Medium?
DMEM/F12 medium is designed to meet the nutritional requirements of a wide range of cell types. It contains essential amino acids, vitamins, inorganic salts, and other components necessary for cell growth and maintenance. The 1:1 mixture ensures a balanced supply of nutrients, making it an ideal choice for both adherent and suspension cultures.
Applications of DMEM/F12 Medium:
DMEM/F12 medium is commonly used in various research and clinical applications, including:
- Primary cell culture
- Stem cell culture
- Transformed cell lines
- Tissue engineering
- Drug screening and toxicity testing
Key Features of DMEM/F12 Medium:
DMEM/F12 medium is formulated to support the growth of a wide range of cell types. It is available in a convenient liquid form, ready to use, and does not require any additional supplementation. The medium is also tested for sterility and performance to ensure consistent results.
Storage and Handling:
DMEM/F12 medium should be stored at 2-8°C and protected from light. It is recommended to use the medium within the specified shelf life to ensure optimal performance. Always follow standard laboratory safety protocols when handling cell culture media.
Risk Note:
This product is intended for laboratory use only. It is not intended for human or animal consumption. Always handle with care and follow standard laboratory safety protocols.
FAQs
What is the shelf life of DMEM/F12 medium?
The shelf life of DMEM/F12 medium is typically 12 months from the date of manufacture when stored at 2-8°C and protected from light.
Can DMEM/F12 medium be used for primary cell culture?
Yes, DMEM/F12 medium is commonly used for primary cell culture. Its balanced nutrient composition supports the growth of a wide range of cell types, including primary cells.
Is DMEM/F12 medium suitable for stem cell culture?
Yes, DMEM/F12 medium is suitable for stem cell culture. Its nutrient-rich formulation supports the growth and maintenance of stem cells.
What is the recommended storage temperature for DMEM/F12 medium?
DMEM/F12 medium should be stored at 2-8°C and protected from light to ensure optimal performance.
Can DMEM/F12 medium be used for tissue engineering applications?
Yes, DMEM/F12 medium is commonly used in tissue engineering applications. Its balanced nutrient composition supports the growth and differentiation of various cell types.
