Description
Specifications Table
Product Name – Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) Quantity/Pack Size – 20 liters Form – Liquid Grade – Cell Culture Grade Application – Cell Culture
Product Overview
Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) is a specialized cell culture medium formulated to support the growth of a wide variety of cell types. This 1:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM) and Ham’s F-12 nutrient mixture provides a balanced combination of essential nutrients, vitamins, amino acids, and inorganic salts necessary for optimal cell growth and proliferation. The medium is designed to maintain physiological pH and osmolarity, ensuring a stable environment for cell cultures. It is particularly suitable for the cultivation of primary cells, stem cells, and cell lines that require a nutrient-rich environment. The high-quality formulation of DMEM/F12 medium makes it an ideal choice for researchers and educators seeking reliable and consistent results in their cell culture experiments.
FAQs
1. What is the primary use of DMEM/F12 Medium?
DMEM/F12 Medium is primarily used for cell culture, supporting the growth and proliferation of various cell types, including primary cells, stem cells, and cell lines.
2. Is this medium suitable for all types of cells?
DMEM/F12 Medium is suitable for a wide range of cell types, particularly those that require a nutrient-rich environment. However, it is always recommended to check the specific requirements of the cell type being cultured.
3. What are the key components of DMEM/F12 Medium?
The key components of DMEM/F12 Medium include essential nutrients, vitamins, amino acids, and inorganic salts, all of which are necessary for optimal cell growth and proliferation.
4. How should DMEM/F12 Medium be stored?
DMEM/F12 Medium should be stored at 2-8°C. It is important to avoid freezing the medium, as this can affect its performance and stability.
5. Can DMEM/F12 Medium be used for long-term cell culture?
Yes, DMEM/F12 Medium is suitable for long-term cell culture. Its balanced formulation ensures a stable environment for cells, supporting their growth and proliferation over extended periods.
