Description
Specifications Table
| Product Name | Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 3:1 Mixture) |
|---|---|
| Quantity/Pack Size | 20 liters |
| Form | Liquid |
| Grade | Cell Culture Grade |
| Application | Cell Culture |
Product Description
Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 3:1 Mixture) is a specialized cell culture medium designed to support the growth and maintenance of a wide range of cell types. This medium is a carefully balanced mixture of Dulbecco’s Modified Eagle Medium (DMEM) and Ham’s F-12 Nutrient Mixture, formulated in a 3:1 ratio to provide the optimal nutrient environment for various cell culture applications.
Why Choose DMEM/F12 Medium?
DMEM/F12 is particularly effective for culturing cells that require a more complex nutrient environment than what is provided by standard DMEM or F-12 media. It is widely used in research laboratories, academic institutions, and biotechnology companies for its versatility and reliability in supporting cell growth and differentiation.
Key Features:
1. Balanced Nutrient Composition: The 3:1 mixture of DMEM and F-12 ensures a balanced supply of essential nutrients, amino acids, vitamins, and minerals.
2. Versatile Applications: Suitable for a variety of cell types, including primary cells, stem cells, and immortalized cell lines.
3. Consistent Performance: High-quality formulation ensures consistent results in cell culture experiments.
4. Sterile and Ready-to-Use: Conveniently packaged in a 20-liter pack size, ready for immediate use in your laboratory.
Applications of DMEM/F12 Medium:
1. Cell Culture: Ideal for maintaining and growing various cell types in vitro.
2. Research: Supports a wide range of research applications, including drug discovery, toxicology studies, and stem cell research.
3. Biotechnology: Used in bioprocessing and biomanufacturing for the production of biologics and other therapeutic products.
How to Use DMEM/F12 Medium:
1. Preparation: DMEM/F12 medium is supplied as a liquid and is ready to use. No additional preparation is required.
2. Storage: Store the medium at 2-8°C. Avoid freezing.
3. Usage: Follow standard cell culture protocols for culturing your specific cell type. Ensure proper aseptic techniques are maintained to prevent contamination.
Safety Information:
Handle with care. Avoid contact with skin and eyes. In case of accidental contact, rinse thoroughly with water. Store in a cool, dry place away from direct sunlight.
FAQs
What is the shelf life of DMEM/F12 medium?
The shelf life of DMEM/F12 medium is typically 12 months from the date of manufacture when stored at 2-8°C.
Can DMEM/F12 medium be used for primary cell culture?
Yes, DMEM/F12 medium is suitable for primary cell culture. Its balanced nutrient composition supports the growth and maintenance of various primary cell types.
What is the recommended storage temperature for DMEM/F12 medium?
DMEM/F12 medium should be stored at 2-8°C. Avoid freezing the medium as it may affect its performance.
Is DMEM/F12 medium suitable for stem cell research?
Yes, DMEM/F12 medium is commonly used in stem cell research due to its ability to support the growth and differentiation of stem cells.
What are the key nutrients in DMEM/F12 medium?
DMEM/F12 medium contains a balanced mix of essential nutrients, including amino acids, vitamins, minerals, and other growth factors necessary for cell culture.
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