Description
Specifications Table
| Product Name | RIPA Buffer | TCL131-500ML | Himedia |
|---|---|
| Quantity/Pack Size | 500 ml |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | Protein extraction, cell lysis, Western blotting |
Product Description
RIPA Buffer (TCL131-500ML) by Himedia is a premium lysis buffer designed for efficient protein extraction from cells and tissues. This high-quality buffer is widely used in research labs for Western blotting, immunoprecipitation, and other molecular biology applications. The formulation ensures effective cell membrane disruption while preserving protein integrity, making it a reliable choice for researchers and students alike.
Whether you’re working on protein analysis or studying cellular pathways, this RIPA buffer provides consistent results. Its balanced composition includes detergents like NP-40 and sodium deoxycholate, along with salts and Tris buffer, to maintain optimal pH and protein stability. The 500 ml pack size is ideal for frequent use in academic and professional settings.
For those looking to buy RIPA buffer online, eqipped offers a seamless shopping experience with authentic Himedia products. Our platform ensures you receive genuine lab supplies with fast delivery across India. Whether you’re a student setting up your first experiment or a seasoned researcher, this buffer is a must-have in your lab inventory.
Need a reliable lysis buffer for your protein extraction experiments? Buy RIPA Buffer 500ml online today and experience hassle-free research.
The versatility of RIPA Buffer (TCL131) makes it suitable for various applications, including:
- Extracting total cellular proteins from cultured cells
- Preparing samples for SDS-PAGE and Western blotting
- Studying protein-protein interactions via co-immunoprecipitation
When handling this buffer, always follow standard lab safety protocols. Store it at room temperature and avoid contamination to ensure long-term stability. For best results, supplement with protease and phosphatase inhibitors before use, depending on your experimental requirements.
FAQs
1. What is RIPA Buffer used for?
RIPA Buffer is primarily used for lysing cells and extracting proteins. It’s ideal for Western blotting, immunoprecipitation, and other protein analysis techniques due to its strong detergent-based formulation.
2. How do I use RIPA Buffer for protein extraction?
Add the buffer directly to cell pellets or tissue samples, incubate on ice for 15-30 minutes, and then centrifuge to collect the protein supernatant. For best results, supplement with protease inhibitors.
3. Can I buy RIPA Buffer online in India?
Yes, you can easily buy RIPA Buffer 500ml online from eqipped. We offer authentic Himedia products with fast delivery across India.
4. What is the shelf life of RIPA Buffer (TCL131-500ML)?
When stored at room temperature in its original packaging, this buffer has a shelf life of 12-24 months. Always check the expiration date on the label.
5. Is this buffer compatible with all cell types?
RIPA Buffer works well with most mammalian cell types. However, for tougher tissues or specific applications, you may need to optimize the lysis conditions or use additional mechanical disruption.
