Description
Specifications Table
| Product Name | EZXpand Colcemid® Human Umbilical Vein Endothelial Cell Culture Kit (w/o VEGF) |
|---|---|
| Quantity/Pack Size | 0.5 million cells/vial |
| Form | Frozen |
| Grade | Research Grade |
| Application | Cell Culture |
Product Description
The EZXpand Colcemid® Human Umbilical Vein Endothelial Cell Culture Kit (w/o VEGF) is a premium kit designed for culturing human umbilical vein endothelial cells (HUVECs) in a VEGF-free environment. This kit is ideal for researchers, educators, and students looking to study endothelial cell behavior and function without the influence of VEGF. The kit includes 0.5 million cells per vial, ensuring a consistent and reliable source of HUVECs for your experiments.
Why Choose This Kit?
This kit is perfect for those who need a VEGF-free environment to study endothelial cell behavior. It is suitable for a variety of applications, including angiogenesis research, drug testing, and cell biology studies. The high-grade cells ensure reproducibility and reliability in your experiments.
How to Use:
Thaw the vial quickly at 37°C in a water bath. Transfer the cells to a culture dish containing the appropriate growth medium. Incubate the cells at 37°C in a humidified atmosphere with 5% CO2. Change the medium every 2-3 days until the cells reach confluence.
Storage:
Store the kit at -80°C until ready for use. Avoid repeated freeze-thaw cycles to maintain cell viability.
Safety Note:
This product is for laboratory use only. Handle with care and follow standard safety protocols for working with biological materials.
Risk Note:
This product may contain hazardous materials. Use appropriate personal protective equipment (PPE) and follow safety guidelines when handling.
FAQs
What is the shelf life of the EZXpand Colcemid® Human Umbilical Vein Endothelial Cell Culture Kit?
The shelf life of the kit is 6 months from the date of manufacture when stored at -80°C.
Can this kit be used for in vivo studies?
This kit is designed for in vitro use only. For in vivo studies, please consult with a specialist to ensure the appropriate kit is selected.
What is the recommended culture medium for this kit?
The recommended culture medium is Endothelial Cell Growth Medium (ECGM) without VEGF.
How do I thaw the cells?
Thaw the vial quickly at 37°C in a water bath. Transfer the cells to a culture dish containing the appropriate growth medium.
What is the passage number of the cells in this kit?
The cells in this kit are at passage 3.
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