Description
Specifications Table
| Product Name | ACES Buffer | Q10011 | Qualigens |
|---|---|
| Quantity/Pack Size | 5g |
| Form | Powder |
| Grade | Biochemical/Molecular Biology |
| Application | Electrophoresis, protein studies, buffer preparation |
Product Description
ACES Buffer (N-(2-Acetamido)-2-aminoethanesulfonic acid) is a versatile biochemical buffer widely used in laboratories for maintaining stable pH conditions. With a pKa of 6.8 at 20°C, it is particularly effective in the pH range of 6.1–7.5, making it ideal for electrophoresis, enzyme assays, and cell culture applications. The Qualigens ACES Buffer (Q10011) is a high-purity powder formulation, ensuring minimal interference in sensitive experiments.
Whether you’re a student setting up a gel electrophoresis experiment or a researcher optimizing protein purification protocols, this buffer provides consistent performance. Its zwitterionic nature reduces ionic interactions, making it a preferred choice for biochemical assays. For those looking to buy ACES buffer online in India, eqipped offers a reliable supply with fast shipping and competitive pricing.
Need a stable buffer for your next experiment? Order ACES Buffer from eqipped today for precise pH control.
Applications of ACES Buffer
ACES Buffer is commonly used in:
- Electrophoresis (SDS-PAGE, native PAGE)
- Protein purification and stabilization
- Enzyme activity assays
- Cell culture media preparation
- Molecular biology protocols requiring pH 7.4
Why ACES Buffer is Essential for Your Lab
Unlike traditional buffers like Tris or phosphate, ACES Buffer offers superior pH stability and minimal metal ion binding, reducing experimental variability. Its compatibility with biological systems makes it a staple in research labs. When you buy ACES buffer online from eqipped, you get a product tested for consistency and purity, ensuring reproducible results every time.
For educators, this buffer is an excellent choice for teaching electrophoresis techniques, as its pH range aligns with common laboratory conditions. Researchers working with sensitive proteins or nucleic acids will appreciate its non-interfering properties.
How to Prepare ACES Buffer Solution
To prepare a 0.1M ACES Buffer solution at pH 7.4:
- Dissolve 1.82g of ACES Buffer powder in 80mL of distilled water.
- Adjust the pH to 7.4 using NaOH or HCl.
- Add distilled water to a final volume of 100mL.
- Sterilize by filtration if required for cell culture use.
Looking for a reliable ACES buffer supplier in India? eqipped delivers lab-grade quality with hassle-free ordering.
Safety and Handling
ACES Buffer is generally non-hazardous but should be handled with standard laboratory precautions. Wear appropriate PPE (gloves, goggles) when preparing solutions. Store in a cool, dry place away from direct sunlight. For disposal, follow local regulations for chemical waste.
FAQs
What is the ideal pH range for ACES Buffer?
ACES Buffer is effective between pH 6.1–7.5, with optimal buffering capacity around pH 6.8–7.4.
Can ACES Buffer be used for cell culture?
Yes, it is commonly used in cell culture media due to its low toxicity and stable pH maintenance.
How does ACES Buffer compare to Tris Buffer?
ACES Buffer has a lower temperature dependence and minimal metal ion binding compared to Tris, making it better for sensitive applications.
What is the shelf life of ACES Buffer powder?
When stored properly in a sealed container, the powder has a shelf life of 2–3 years.
Where can I buy ACES Buffer online in India?
You can purchase Qualigens ACES Buffer (Q10011) from eqipped with fast delivery across India.
