Description
Specifications Table
Product Name – Ninhydrin ER
Quantity/Pack Size – 10 GM, 25 GM
Form – Crystalline Powder
Grade – ER (Extra Pure)
Application – Amino acid detection, Chromatography, Peptide analysis
Product Overview
Ninhydrin ER stands as an indispensable high-purity chemical reagent, a cornerstone for a wide array of biochemical and analytical applications in modern laboratories. Scientifically identified as 2,2-Dihydroxyindane-1,3-dione, its primary renown stems from its highly specific and distinct reaction with amino acids, peptides, and proteins. Upon gentle heating, Ninhydrin ER reacts to form a vivid deep blue or purple compound, famously known as Ruhemann’s purple. This characteristic color change makes it an unparalleled tool for both the qualitative identification and quantitative estimation of these vital biomolecules. The Extra Pure (ER) grade classification guarantees superior quality, exceptional consistency, and unwavering reliability, which are paramount for obtaining accurate and reproducible results in any meticulous scientific endeavor.
This versatile reagent’s utility spans numerous scientific disciplines. In chromatography, it serves as a crucial visualizing agent for amino acids separated on paper or thin-layer plates, revealing their presence with clarity. Its applications extend to forensic science, where it is effectively employed in developing latent fingerprints by reacting with amino acid residues. Researchers across biochemistry, molecular biology, and proteomics depend on Ninhydrin ER for precise amino acid analysis during protein sequencing, metabolic pathway studies, and general protein characterization. For students and educators, it is an essential chemical for demonstrating fundamental organic and biochemistry principles, offering hands-on experience with colorimetric reactions. The inherent stability and high purity of eqipped’s Ninhydrin ER ensure optimal performance, providing the consistent reactivity required for even the most sensitive analytical procedures. This reagent is truly a fundamental component for any laboratory dedicated to the study and analysis of amino acid chemistry, underpinning robust scientific inquiry and effective educational exploration.
FAQs
1. How does Ninhydrin ER facilitate amino acid analysis in laboratories?
Ninhydrin ER reacts specifically with amino acids, peptides, and proteins to produce a colored compound, which allows for their visual detection and spectrophotometric quantification, crucial for analytical procedures.
2. What are the key features of the ER grade of Ninhydrin?
The ER (Extra Pure) grade signifies a high level of purity, ensuring minimal impurities that could interfere with reactions, leading to more accurate and reliable experimental results in sensitive applications.
3. Can Ninhydrin ER be used in thin-layer chromatography (TLC)?
Yes, Ninhydrin ER is a widely used spray reagent in thin-layer chromatography for the visualization of amino acids and other primary amines separated on TLC plates.
4. What is the shelf life of Ninhydrin ER when stored correctly?
When stored in optimal conditions—cool, dry, and sealed—Ninhydrin ER typically has a long shelf life, but it’s always recommended to check the specific expiration date on the product label for best results.
5. Is Ninhydrin ER compatible with all types of samples containing amino acids?
Ninhydrin ER reacts broadly with primary amines, including most alpha-amino acids. However, specific sample matrices might require preliminary purification steps to avoid interference from other reactive compounds.
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