Description
Specifications Table
Product Name – Whatman NC10 Membrane Filter
Quantity/Pack Size – 47MM, 0.1uM (Pack of 100) | 50MM, 0.1uM (Pack of 100)
Form – Circular Disc
Grade – NC10 (Nitrocellulose)
Application – Sterile filtration, particle retention, microbial analysis
Product Overview
The Whatman NC10 Membrane Filter is engineered for high-efficiency particle retention and sterile filtration in laboratory settings. Constructed from pure nitrocellulose, this filter delivers consistent 0.1?m pore size retention, ensuring reliable separation of microorganisms, fine particles, and colloidal substances. Its uniform porosity and smooth surface minimize sample loss while maximizing flow rates, making it ideal for applications requiring precision and reproducibility. The hydrophobic nature of nitrocellulose provides excellent chemical compatibility with aqueous solutions, alcohols, and mild organic solvents, though it is not suitable for strong acids or bases. Each filter undergoes stringent quality control to guarantee lot-to-lot consistency, with low extractables to prevent sample contamination. The white, opaque membrane allows for easy visual inspection of retained particles, while its mechanical strength ensures durability during handling and processing. Available in 47MM and 50MM diameters, these filters are pre-sterilized (gamma-irradiated) to meet aseptic workflow requirements, eliminating the need for autoclaving. Whether used in syringe filtration, vacuum manifolds, or gravity-fed systems, the Whatman NC10 provides a balance of high loading capacity and fast filtration speeds, reducing processing time without compromising performance. Its compatibility with standard filtration apparatus makes integration seamless across diverse lab setups.
FAQs
1. What is the chemical resistance profile of Whatman NC10 membrane filters?
Whatman NC10 filters are compatible with water, alcohols, and mild organic solvents like acetone or ethyl acetate. Avoid exposure to strong acids, bases, or halogenated solvents, as these can degrade the nitrocellulose matrix. For aggressive solvents, consider PTFE-based alternatives.
2. Can these filters be autoclaved for sterilization?
Autoclaving is not recommended for NC10 membranes, as the combination of heat and moisture can compromise the nitrocellulose structure. The filters are pre-sterilized via gamma irradiation, ensuring they meet aseptic standards straight out of the pack.
3. How does the 0.1?m pore size compare to 0.2?m filters in microbial retention?
A 0.1?m pore size retains smaller particles, including mycoplasmas and some viruses, which may pass through 0.2?m filters. However, flow rates are slower due to tighter pore distribution. Choose 0.1?m for stringent sterility requirements or when targeting sub-micron contaminants.
4. Are these filters suitable for protein binding studies?
Nitrocellulose membranes like NC10 exhibit moderate protein binding due to hydrophobic interactions. For low-binding applications, consider cellulose acetate or PES membranes. If protein adsorption is desired (e.g., dot blots), NC10 can be used with appropriate blocking agents.
5. What?s the shelf life of unopened Whatman NC10 filters?
When stored in original packaging at room temperature (15?30?C) and protected from humidity, the filters retain their performance for up to 5 years. Avoid exposure to direct sunlight or temperature fluctuations, as these can affect pore integrity over time.
