Description
Specifications Table
Product Name – Whatman NC45 Membrane Filter
Quantity/Pack Size – 25MM, 0.45uM (Pack of 100), 47MM, 0.45uM (Pack of 100), 50MM, 0.45uM (Pack of 100), 85MM, 0.45uM (Pack of 50), 90MM, 0.45uM (Pack of 50), 100MM, 0.45uM (Pack of 50), 110MM, 0.45uM (Pack of 50), 142MM, 0.45uM (Pack of 25)
Form – Circular membrane discs
Grade – Nitrocellulose (NC45)
Application – Protein binding, nucleic acid blotting, general lab filtration
Product Overview
The Whatman NC45 Membrane Filter is a high-performance nitrocellulose membrane designed for critical laboratory applications requiring superior protein binding and nucleic acid retention. Engineered with a uniform 0.45?m pore size, this membrane ensures consistent flow rates and high binding capacity, making it ideal for Western blotting, dot blotting, and general filtration tasks. The nitrocellulose composition provides excellent mechanical strength and chemical compatibility, allowing for reliable performance across diverse protocols. Each membrane undergoes stringent quality control to guarantee uniformity, minimizing background noise and maximizing signal clarity in downstream applications. The hydrophobic nature of nitrocellulose enhances protein adsorption, while the optimized pore structure facilitates efficient liquid passage without clogging. Available in multiple diameters to suit various experimental setups, these filters are compatible with standard filtration apparatus and blotting systems. Their sterile packaging maintains purity during storage, ensuring ready-to-use convenience for time-sensitive procedures. The NC45 grade specifically offers balanced protein retention and flow characteristics, making it a versatile choice for both routine and specialized laboratory workflows.
FAQs
1. What is the protein binding capacity of Whatman NC45 membranes?
These membranes typically bind 80-100 ?g/cm? of protein, depending on the specific protein and binding conditions.
2. Can these filters be used for nucleic acid transfer applications?
Yes, the NC45 grade is suitable for Southern and Northern blotting applications due to its nucleic acid retention properties.
3. What solvents are compatible with these nitrocellulose membranes?
They are compatible with most aqueous solutions and mild organic solvents, but avoid strong acids or bases that may degrade the membrane.
4. How should these membranes be stored for long-term use?
Store in a cool, dry place in their original packaging to prevent contamination and maintain performance.
5. Are these filters autoclavable for sterile applications?
No, nitrocellulose membranes cannot be autoclaved as high temperatures will damage the membrane structure.
