Description
Specifications Table
| Product Name | M.T.T. Tetrazolium AR 98.0% |
|---|---|
| Pack Size | 100 mg, 250 mg, 1 gm, 5 gm |
| Form | Powder |
| Grade | AR (Analytical Reagent) |
| Application | Cell viability assays, cytotoxicity studies, biochemical research |
Product Description
M.T.T. Tetrazolium (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) is a widely used reagent in cell biology and biochemistry for assessing cell viability and proliferation. This high-purity AR-grade MTT powder from CDH ensures reliable results in research applications, making it a staple in laboratories across India.
Whether you’re a student conducting experiments or a researcher validating cellular responses, this MTT tetrazolium salt provides consistent performance. Its yellowish powder form dissolves easily in aqueous solutions, forming a water-soluble tetrazolium dye that gets reduced by metabolically active cells into insoluble purple formazan crystals. The intensity of this color change directly correlates with cell viability, offering quantitative data for your studies.
At eqipped, we understand the importance of quality reagents in scientific work. That’s why we source this MTT assay reagent from trusted manufacturers, ensuring you receive a product that meets stringent purity standards. Whether you’re setting up an MTT assay kit or need bulk quantities for ongoing research, our range of pack sizes (100 mg to 5 gm) caters to different experimental scales.
Need MTT powder for your next cell viability test? Order now and get authentic CDH quality delivered to your lab.
How MTT Assay Works
The MTT assay is based on the reduction of yellow MTT tetrazolium salt to purple formazan crystals by mitochondrial dehydrogenases in living cells. This process only occurs in viable cells with active metabolism, making it an excellent indicator of cell health. The resulting formazan can be solubilized and quantified using a spectrophotometer, providing objective data for your research.
This method is particularly valuable for:
- Drug toxicity screening
- Cytotoxicity assessments
- Cell proliferation studies
- Antimicrobial activity testing
Proper Handling and Storage
To maintain the integrity of your MTT tetrazolium salt:
- Store in a cool, dry place away from direct sunlight
- Keep the container tightly sealed when not in use
- Avoid exposure to moisture which can affect reagent performance
- Use appropriate personal protective equipment when handling
For best results, prepare fresh MTT solutions for each experiment and follow standard assay protocols. The reagent should be protected from light during both storage and experimental procedures to prevent degradation.
Applications in Research
This high-quality MTT chemical finds applications across various scientific disciplines:
In cancer research, it’s used to evaluate the efficacy of potential chemotherapeutic agents by measuring their impact on tumor cell viability. Microbiologists employ it to assess bacterial growth inhibition by antimicrobial compounds. Pharmacologists use MTT assays to screen drug candidates for cytotoxicity before advancing to animal studies.
For educators, this reagent provides an excellent tool for demonstrating cellular metabolism concepts in practical laboratory sessions. The visual color change makes it particularly effective for teaching purposes while maintaining scientific rigor.
Why Researchers Trust This Product
The AR grade designation indicates this MTT powder meets analytical reagent standards, ensuring minimal interference from impurities in your experiments. CDH’s reputation for quality laboratory chemicals makes this product a reliable choice for both academic and industrial research settings.
When you buy MTT tetrazolium salt from eqipped, you’re getting a product that has been carefully selected for its consistency and performance. Our streamlined ordering process and nationwide delivery ensure you can focus on your research without supply chain worries.
Ready to enhance your cell viability assays? Shop MTT assay reagent online now for genuine CDH quality.
Safety Considerations
While MTT is generally considered safe for laboratory use, proper handling procedures should be followed:
- Wear appropriate protective clothing, gloves, and eye protection
- Work in a well-ventilated area or fume hood
- Avoid inhalation or direct contact with skin
- Dispose of waste according to local regulations
FAQs
What is the shelf life of MTT tetrazolium salt?
When stored properly in a cool, dry place away from light, MTT powder typically has a shelf life of 2-3 years. Always check the manufacturer’s expiration date on the packaging.
Can I use this MTT powder for both mammalian and bacterial cells?
Yes, this MTT assay reagent is suitable for both mammalian cell lines and bacterial cultures. However, you may need to optimize the protocol for different cell types.
What’s the difference between MTT and other tetrazolium salts like XTT or WST-1?
MTT forms insoluble formazan crystals that require solubilization, while XTT and WST-1 produce water-soluble formazan. MTT is generally more sensitive but requires an additional solubilization step.
How should I prepare the MTT solution for my assay?
A common protocol involves dissolving MTT powder in PBS or culture medium at 5 mg/mL, followed by sterile filtration. The exact concentration may vary based on your specific assay requirements.
Is this MTT chemical suitable for high-throughput screening?
Yes, this high-purity MTT tetrazolium salt is excellent for high-throughput applications. Its consistent performance makes it ideal for automated assay systems in drug discovery and toxicity screening.
