Description
Specifications Table
| Product Name | Ponceau S for Electrophoresis C.I. No. 27195 | 314915 | CDH |
|---|---|
| Pack Size | 25gm, 100gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Protein gel staining, electrophoresis |
Product Description
Ponceau S is a widely used dye in molecular biology for staining proteins on nitrocellulose or PVDF membranes after electrophoresis. Unlike Coomassie stains, Ponceau S offers rapid, reversible staining, making it ideal for quick protein visualization before immunoblotting. Its deep red color provides excellent contrast, ensuring clear detection of protein bands.
This high-purity dye is specifically formulated for laboratory use, ensuring consistent results in protein gel staining applications. Whether you’re a student conducting experiments or a researcher validating protein transfer, Ponceau S from eqipped delivers reliable performance.
Need a dependable protein stain for your electrophoresis experiments? Shop Ponceau S online today for fast, accurate results.
Ponceau S works by binding to amino groups in proteins, allowing for temporary staining that can be easily destained if needed. It’s particularly useful for verifying protein transfer efficiency in Western blotting workflows. The dye is compatible with most electrophoresis buffers and does not interfere with downstream immunodetection steps.
For best results, prepare a 0.1% (w/v) solution in 5% acetic acid. Staining typically takes just a few minutes, and destaining can be achieved with water or a mild acetic acid solution. This makes Ponceau S a time-saving alternative to other protein stains.
FAQs
What is Ponceau S used for in electrophoresis?
Ponceau S is used to stain proteins on membranes (nitrocellulose/PVDF) after electrophoresis. It helps visualize protein bands before immunoblotting.
Is Ponceau S staining reversible?
Yes, Ponceau S staining is reversible. The dye can be easily washed off with water or mild acetic acid without affecting protein integrity.
How do I prepare Ponceau S staining solution?
Dissolve 0.1g of Ponceau S in 100ml of 5% acetic acid. Filter if necessary before use.
Can Ponceau S interfere with Western blotting?
No, Ponceau S does not interfere with antibody binding in Western blotting. It’s commonly used to confirm protein transfer before immunodetection.
Where can I buy Ponceau S online in India?
You can buy Ponceau S for electrophoresis online at eqipped. We offer fast shipping and high-quality lab supplies for students and researchers.
