Description
Specifications Table
| Product Name | o-Nitro Phenyl-β-D-Galactopyranoside 98.0% |
|---|---|
| Pack Size | 100g |
| Form | Powder |
| Grade | 98.0% |
| Application | β-galactosidase substrate, biochemical assays |
Product Description
o-Nitrophenyl β-D-galactopyranoside (ONPG) is a high-purity substrate widely used in biochemical and molecular biology laboratories for detecting β-galactosidase activity. This compound is essential for colorimetric assays, where the enzyme hydrolyzes ONPG to produce a yellow-colored product (o-nitrophenol), allowing for easy quantification of enzymatic activity.
With a purity of 98.0%, this ONPG powder ensures reliable and consistent results in research applications. Whether you’re conducting bacterial lacZ gene expression studies or enzymatic activity measurements, this substrate provides the sensitivity and accuracy needed for precise data.
At eqipped, we understand the importance of quality reagents in scientific research. That’s why we source our ONPG substrate from trusted manufacturers, ensuring you receive a product that meets the highest standards for your experiments.
Looking to buy o-Nitrophenyl β-D-galactopyranoside online? eqipped offers a seamless purchasing experience with fast shipping across India, making it easier for students, researchers, and educators to access premium lab supplies.
Applications of ONPG Substrate
ONPG is a versatile reagent with several key applications in laboratory settings:
1. β-Galactosidase Assays: Used to measure the activity of β-galactosidase in bacterial and eukaryotic cells, particularly in lacZ gene expression studies.
2. Microbial Identification: Helps in identifying lactose-fermenting bacteria by detecting β-galactosidase production.
3. Enzyme Kinetics: Employed in kinetic studies to determine the reaction rates of β-galactosidase under different conditions.
4. Molecular Cloning: Used in blue-white screening for recombinant DNA technology, where β-galactosidase activity indicates successful cloning.
For researchers and students working on projects involving gene expression or enzymatic activity, ONPG is an indispensable tool. Its stability and ease of use make it a preferred choice in both academic and industrial labs.
Why ONPG is Essential for Your Lab
When you buy ONPG powder online, you’re investing in a reagent that offers several advantages:
High Sensitivity: The yellow color produced upon hydrolysis is easily detectable, even at low enzyme concentrations.
Stability: ONPG remains stable under standard storage conditions, ensuring long shelf life and consistent performance.
Versatility: Compatible with various assay formats, including spectrophotometric and microplate-based methods.
Cost-Effective: Provides reliable results without the need for expensive equipment, making it ideal for budget-conscious labs.
Whether you’re setting up a new lab or restocking your supplies, eqipped makes it easy to buy o-Nitrophenyl β-D-galactopyranoside online with just a few clicks. Our user-friendly platform ensures a hassle-free shopping experience, so you can focus on what matters most—your research.
Safety and Handling
While ONPG is generally safe to handle, it’s important to follow standard laboratory practices:
Storage: Keep the container tightly closed in a cool, dry place. Store away from incompatible substances.
Handling: Wear appropriate personal protective equipment (PPE), such as gloves and safety goggles, when handling the powder.
Disposal: Dispose of waste material in accordance with local regulations for chemical waste.
For detailed safety information, refer to the Safety Data Sheet (SDS) provided with the product.
How to Use ONPG in Your Experiments
Using ONPG in your assays is straightforward. Here’s a simple guide to get you started:
1. Prepare the Substrate Solution: Dissolve ONPG in a suitable buffer (e.g., phosphate buffer) to the desired concentration.
2. Add to Your Sample: Mix the substrate solution with your enzyme or cell lysate.
3. Incubate: Allow the reaction to proceed at the optimal temperature for β-galactosidase activity.
4. Measure Absorbance: Use a spectrophotometer to measure the absorbance of the yellow product at 420 nm.
For best results, always follow the protocol specific to your experiment. If you’re new to using ONPG, consult your lab manual or supervisor for detailed instructions.
Buy ONPG Online at eqipped
When you choose eqipped for your lab supplies, you’re choosing a partner committed to quality and convenience. Our platform is designed to meet the needs of students, researchers, and educators across India, offering a wide range of products at competitive prices.
Ready to buy o-Nitrophenyl β-D-galactopyranoside online? Explore our selection today and experience the ease of shopping with eqipped. With fast shipping and dedicated customer support, we ensure your lab is always well-stocked and ready for your next breakthrough.
FAQs
What is the purpose of ONPG in β-galactosidase assays?
ONPG serves as a substrate for β-galactosidase, producing a yellow-colored product (o-nitrophenol) upon hydrolysis. This color change allows researchers to measure enzyme activity spectrophotometrically.
How should I store ONPG powder?
Store ONPG in a tightly sealed container in a cool, dry place, away from moisture and direct sunlight. Proper storage ensures long-term stability and performance.
Can ONPG be used in microbial identification?
Yes, ONPG is commonly used to identify lactose-fermenting bacteria by detecting β-galactosidase production, which is a key trait in microbial classification.
What is the shelf life of ONPG powder?
When stored under recommended conditions, ONPG powder typically has a shelf life of 2-3 years. Always check the expiration date on the product label.
Is ONPG suitable for blue-white screening in molecular cloning?
Absolutely. ONPG is a critical component in blue-white screening, where β-galactosidase activity helps distinguish between recombinant and non-recombinant colonies.
