Description
Specifications Table
Product Name – 3,3,5,5-Tetramethylbenzidine (TMB) For Molecular Biology 99.0%
Quantity/Pack Size – 1 gm, 5 gm
Form – Powder
Grade – Molecular Biology
Application – ELISA, Immunoassays, Research
Product Overview
3,3,5,5-Tetramethylbenzidine (TMB) is a high-purity substrate widely used in molecular biology for its exceptional sensitivity in colorimetric assays. This 99.0% grade TMB is meticulously purified to ensure reliable performance in ELISA, Western blotting, and other immunoassays. Its stable and consistent formulation makes it ideal for researchers and educators requiring precise and reproducible results. The compound’s ability to produce a deep blue color upon oxidation, which can be converted to a stable yellow end product, enhances its utility in quantitative analyses. Manufactured under stringent quality control measures, this TMB is free from contaminants that could interfere with assay accuracy. Its versatility and high purity make it a trusted choice for laboratories engaged in cutting-edge research and educational institutions conducting advanced experiments.
FAQs
1. What is the primary use of 3,3,5,5-Tetramethylbenzidine (TMB)?
3,3,5,5-Tetramethylbenzidine (TMB) is primarily used as a substrate in colorimetric assays, particularly in ELISA and Western blotting, to detect peroxidase activity.
2. Is this TMB suitable for educational purposes?
Yes, this high-purity TMB is suitable for educational purposes, including lab experiments and training in molecular biology techniques.
3. How should 3,3,5,5-Tetramethylbenzidine (TMB) be stored?
Store TMB in a cool, dry place away from light and moisture to maintain its stability and purity.
4. Can TMB be used in other types of assays besides ELISA?
Yes, TMB can be used in various immunoassays and research applications beyond ELISA, including immunohistochemistry and dot blotting.
5. What is the significance of the 99.0% purity grade?
The 99.0% purity grade ensures minimal interference from contaminants, leading to more accurate and reproducible assay results.










