Description
Specifications Table
| Product Name | Coomassie Brilliant Blue R 250 C.I. 42660 HISURE | H10245 | CDH |
|---|---|
| Pack Size | 5 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Protein staining in SDS-PAGE, gel electrophoresis |
Product Description
Coomassie Brilliant Blue R 250 is a widely used dye in biochemistry and molecular biology for staining proteins in polyacrylamide gels. Its deep blue color binds non-specifically to proteins, making it an essential tool for visualizing protein bands after SDS-PAGE. Whether you’re a student, researcher, or educator, this high-quality dye ensures clear and reliable results in your experiments.
This product is sourced from CDH, a trusted name in laboratory chemicals, and is packaged in a convenient 5 gm pack. The dye is highly soluble in water and methanol, making it easy to prepare staining solutions. Its sensitivity allows for the detection of proteins at concentrations as low as 0.1 µg, making it ideal for both qualitative and quantitative analysis.
When you buy Coomassie Brilliant Blue R 250 online from eqipped, you get a product that meets stringent quality standards. Our platform ensures that you receive authentic, lab-grade chemicals with fast and reliable delivery across India. Whether you’re setting up a new lab or restocking your supplies, this dye is a must-have for any protein electrophoresis workflow.
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How to Use Coomassie Brilliant Blue R 250
Using this dye for protein staining is straightforward. Here’s a quick guide to help you get started:
- Prepare a staining solution by dissolving the dye in a mixture of methanol, acetic acid, and water. A common recipe is 0.1% Coomassie Brilliant Blue R 250, 50% methanol, and 10% acetic acid.
- After running your SDS-PAGE gel, carefully remove it from the electrophoresis apparatus and place it in the staining solution. Allow it to stain for at least 30 minutes to ensure proper protein binding.
- Once stained, destain the gel using a solution of methanol and acetic acid to remove excess dye. This step enhances the visibility of protein bands.
- Visualize the protein bands under standard lighting. For documentation, you can use a gel imaging system or simply photograph the gel.
This method is widely used in research labs and educational institutions due to its simplicity and effectiveness. If you’re looking for the best Coomassie Brilliant Blue R 250 brand, this product from CDH is a top choice among professionals.
Applications of Coomassie Brilliant Blue R 250
Coomassie Brilliant Blue R 250 is versatile and finds applications in various laboratory settings:
- Protein detection in SDS-PAGE gels.
- Quantitative analysis of proteins using densitometry.
- Educational demonstrations in biochemistry and molecular biology courses.
- Research applications requiring protein visualization.
Its ability to bind to a wide range of proteins makes it indispensable in both academic and industrial research. If you’re searching for where to buy Coomassie Brilliant Blue R 250, eqipped offers a seamless online shopping experience with competitive pricing and genuine products.
Safety and Handling
While Coomassie Brilliant Blue R 250 is generally safe to handle, it’s important to follow standard laboratory safety protocols:
- Wear appropriate personal protective equipment (PPE), including gloves and safety goggles.
- Avoid inhaling the powder or allowing it to come into contact with skin and eyes.
- Store the dye in a cool, dry place away from direct sunlight.
- Dispose of waste solutions according to local regulations for chemical waste.
By adhering to these guidelines, you can ensure safe and effective use of this dye in your experiments.
Why Coomassie Brilliant Blue R 250 is a Lab Essential
This dye has stood the test of time as a go-to reagent for protein staining. Its affordability, ease of use, and reliability make it a staple in labs worldwide. Whether you’re a student learning the basics of gel electrophoresis or a researcher conducting advanced protein analysis, Coomassie Brilliant Blue R 250 delivers consistent results.
At eqipped, we understand the importance of having access to high-quality lab supplies. That’s why we offer this product at competitive prices, ensuring that you get the best value for your money. When you buy Coomassie Brilliant Blue R 250 online from us, you’re choosing a supplier that prioritizes quality and customer satisfaction.
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FAQs
What is Coomassie Brilliant Blue R 250 used for?
Coomassie Brilliant Blue R 250 is primarily used for staining proteins in SDS-PAGE gels. It binds to proteins, making them visible as blue bands, which is essential for analyzing protein samples in research and education.
How do I prepare a staining solution with Coomassie Brilliant Blue R 250?
To prepare a staining solution, dissolve 0.1% of the dye in a mixture of 50% methanol and 10% acetic acid. Stir well until the dye is completely dissolved, and then use it to stain your gel.
Can I use Coomassie Brilliant Blue R 250 for quantitative analysis?
Yes, this dye can be used for quantitative analysis. After staining, you can use densitometry to measure the intensity of protein bands, which correlates with the amount of protein present.
Is Coomassie Brilliant Blue R 250 safe to handle?
While generally safe, it’s important to handle this dye with care. Wear gloves and safety goggles, and avoid inhaling the powder. Follow standard lab safety protocols to minimize any risks.
Where can I buy Coomassie Brilliant Blue R 250 online in India?
You can buy Coomassie Brilliant Blue R 250 online from eqipped. We offer genuine, lab-grade products with fast and reliable delivery across India. Order now to get your supplies delivered to your doorstep.
