Description
Specifications Table
Product Name – Diaphorase Source : CI. Kluyveri Cell Culture Tested
Quantity/Pack Size – 1 ku
Form – Lyophilized Powder
Grade – Cell Culture Tested
Application – Enzymatic Assays, Redox Studies, Diagnostics, Biosensors
Product Overview
Diaphorase from Clostridium Kluyveri, offered by eqipped, is a high-purity enzyme meticulously prepared to meet the stringent demands of modern biochemical research and diagnostic applications. This enzyme is specifically validated as “Cell Culture Tested,” ensuring its suitability for sensitive biological systems where minimal interference and consistent performance are paramount. Derived from Clostridium Kluyveri, this Diaphorase exhibits robust activity in transferring electrons from NADH or NADPH to various artificial electron acceptors, making it an indispensable tool for a wide array of enzymatic assays. Its role in spectrophotometric assays, particularly in coupling with other enzymes for quantifying substrates or products, is highly valued. Researchers depend on its reliable performance for studies involving redox reactions, metabolism, and diagnostic kits. The lyophilized powder form ensures excellent stability and ease of handling, allowing for precise reconstitution and consistent results across experiments. With a focus on delivering uncompromised quality, eqipped’s Diaphorase from Clostridium Kluyveri is the preferred choice for scientists seeking an enzyme that provides both specificity and high activity for their critical investigations.
FAQs
1. What are the primary applications of Diaphorase from Clostridium Kluyveri?
Diaphorase from Clostridium Kluyveri is widely used in biochemical assays, especially for quantifying NADH or NADPH. It’s crucial in coupled enzyme reactions, diagnostics, biosensor development, and studies involving redox metabolism due to its ability to transfer electrons efficiently.
2. How should this Diaphorase enzyme be stored to maintain its activity?
For optimal stability and prolonged activity, this lyophilized Diaphorase should be stored at -20°C. Once reconstituted, it is recommended to store aliquots at -20°C or below and avoid repeated freeze-thaw cycles.
3. What does “Cell Culture Tested” mean for this Diaphorase?
“Cell Culture Tested” indicates that this Diaphorase has undergone rigorous quality control to ensure it does not contain contaminants that could interfere with or harm mammalian or bacterial cell cultures, making it suitable for assays involving living cells.
4. Can this Diaphorase be used in conjunction with other enzymes for complex assays?
Yes, Diaphorase is frequently used as a coupling enzyme in multi-enzyme systems. It is highly effective in regenerating cofactors or facilitating detection steps in assays where NADH or NADPH production/consumption is a critical indicator.
5. Is the purity of this Clostridium Kluyveri Diaphorase suitable for quantitative analysis?
Absolutely. This Diaphorase from Clostridium Kluyveri is prepared to a high purity level, which is essential for accurate and reliable quantitative analyses. Its specificity and lack of interfering side activities ensure precise results in sensitive experiments.
