Description
Specifications Table
Product Name – Ethidium Bromide Solution 95.0% (10mg/ml) For Molecular Biology
Quantity/Pack Size – 10 ml
Form – Solution
Grade – 95.0% (10mg/ml), For Molecular Biology
Application – DNA Staining, Gel Electrophoresis, Nucleic Acid Visualization
Product Overview
Ethidium Bromide Solution 95.0% (10mg/ml) is a paramount reagent meticulously prepared for precision in molecular biology. This high-purity solution, provided at an optimal concentration of 10mg/ml, is specifically engineered to facilitate clear and distinct visualization of nucleic acids, including DNA and RNA, within agarose and polyacrylamide gels. Its robust and reliable performance ensures that researchers, students, and educators can confidently detect and analyze target fragments with exceptional clarity and sensitivity. The precisely controlled 95.0% purity guarantees minimal interference from impurities, leading to more accurate, reproducible, and trustworthy experimental outcomes across various demanding laboratory settings.
As an indispensable tool, this Ethidium Bromide solution significantly enhances the readability of gel electrophoresis results, enabling users to effortlessly identify DNA bands under UV illumination. Its consistent quality makes it a highly preferred choice for a broad spectrum of critical molecular biology applications. These include, but are not limited to, detailed DNA fingerprinting, comprehensive PCR product analysis, precise restriction fragment length polymorphism (RFLP) studies, and meticulous northern and southern blotting procedures. The ready-to-use liquid form offers unparalleled convenience and significantly reduces preparation time, allowing scientists and aspiring researchers to allocate more focus to their core experimental work and data interpretation. Manufactured under stringent quality control standards, our Ethidium Bromide Solution consistently delivers the high level of performance and dependability required for the most demanding molecular biology techniques, ensuring reliable and impactful results for your crucial experiments.
FAQs
1.What is the primary application of this Ethidium Bromide Solution?
This solution is primarily used for the visualization of DNA and RNA in agarose and polyacrylamide gels during gel electrophoresis, making nucleic acid bands visible under UV light.
2.How should Ethidium Bromide Solution be safely stored?
It should be stored in a cool, dark place, away from light exposure, and kept in a tightly sealed container to maintain its stability and effectiveness over time.
3.Is this solution compatible with all types of gel electrophoresis?
Yes, this Ethidium Bromide solution is broadly compatible with standard agarose and polyacrylamide gel electrophoresis techniques for nucleic acid separation and detection.
4.What is the recommended working concentration for DNA staining?
Typically, a working concentration of 0.5 µg/ml in the gel or electrophoresis buffer is effective, though specific protocols may vary slightly based on experimental requirements.
5.Are there specific safety precautions when handling Ethidium Bromide?
Yes, Ethidium Bromide is a known mutagen. Always wear appropriate personal protective equipment (PPE), including gloves and a lab coat, and handle it in a designated area with proper waste disposal procedures.
