Description
Specifications Table
Product Name – Iso Amyl Alcohol for Molecular Biology 99.5%
Quantity/Pack Size – 500 ml
Form – Liquid
Grade – Molecular Biology Grade
Application – DNA/RNA Extraction, Anti-foaming Agent, Organic Synthesis
Product Overview
Iso Amyl Alcohol (Isopentyl Alcohol) is a colorless liquid with a characteristic odor, highly valued for its critical role in diverse laboratory procedures, particularly in molecular biology and general chemical synthesis. This 99.5% pure, molecular biology grade reagent is meticulously prepared to meet the stringent demands of sensitive assays. Its exceptional purity ensures consistent, reliable results, effectively minimizing interference from impurities that could compromise experimental integrity and data accuracy, crucial for sensitive research applications.
Within molecular biology, Iso Amyl Alcohol is indispensable during nucleic acid purification, especially in phenol-chloroform extraction protocols. It primarily functions as an effective anti-foaming agent, preventing stable emulsion formation which can hinder clear separation of aqueous and organic phases. This crucial action significantly improves the efficiency of DNA and RNA recovery, leading to higher yields and cleaner preparations. These pure nucleic acids are essential for accurate downstream applications like PCR, sequencing, cloning, and gene expression studies, ensuring robust experimental outcomes.
Beyond nucleic acid extraction, Iso Amyl Alcohol is a versatile organic solvent utilized extensively in various chemical syntheses, esterifications, and as a solvent for fats, resins, and essential oils. Its carefully controlled quality and consistent chemical profile make it highly suitable for demanding research and educational environments where precision, reproducibility, and reagent integrity are paramount. Scientists and educators rely on its unwavering performance for preparing buffers, running analytical assays, and conducting experiments requiring a high-grade primary alcohol. The inherent stability and favorable chemical properties of this reagent underscore its value as a foundational component in numerous laboratory settings, contributing significantly to accurate and reproducible scientific outcomes.
FAQs
1.
What is the primary role of Iso Amyl Alcohol in molecular biology?
Iso Amyl Alcohol primarily functions as an anti-foaming agent and helps prevent emulsion formation during phenol-chloroform DNA/RNA extraction, ensuring clear phase separation for efficient nucleic acid recovery.
2.
Is this Iso Amyl Alcohol suitable for sensitive biochemical applications?
Yes, its molecular biology grade and 99.5% purity make it highly suitable for sensitive biochemical applications where reagent integrity and absence of contaminants are crucial.
3.
How should Iso Amyl Alcohol be stored to maintain its efficacy?
It should be stored in a tightly sealed container in a cool, well-ventilated area, away from direct sunlight, heat, and ignition sources to prevent degradation and ensure long-term stability.
4.
Can Iso Amyl Alcohol be used as a general-purpose solvent?
While primarily known for its role in molecular biology, its properties as an organic solvent allow for its use in various chemical syntheses and as a solvent for fats, resins, and certain oils.
5.
What distinguishes molecular biology grade Iso Amyl Alcohol from other grades?
Molecular biology grade signifies stringent purity standards, tested for minimal impurities that could interfere with nucleic acid isolation or downstream enzymatic reactions, making it ideal for sensitive biological research.
