Description
Specifications Table
| Product Name | Polyethylene Glycol 6000 For Molecular Biology | 971920 | CDH |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Molecular Biology |
| Application | Protein crystallization, PCR, cell fusion |
Product Description
Polyethylene Glycol 6000 (PEG 6000) is a high-molecular-weight polymer widely used in molecular biology and biochemistry laboratories. This versatile compound serves as a precipitating agent in protein purification, a fusion promoter in hybridoma technology, and a stabilizing agent in PCR reactions. Its inert nature and water solubility make it ideal for sensitive biological applications where consistency and purity are critical.
Whether you’re working on protein crystallization, DNA extraction, or cell culture experiments, this molecular biology-grade PEG 6000 from CDH ensures reliable performance. The 500 gm pack size offers excellent value for research labs and educational institutions conducting frequent experiments.
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In molecular biology workflows, PEG 6000 plays several key roles: – Facilitates protein precipitation by reducing solubility – Enhances cell membrane fusion in hybridoma production – Acts as a crowding agent to mimic cellular environments – Stabilizes enzymes during storage and reactions
The CDH brand guarantees consistent batch-to-batch quality, making it a preferred choice among Indian researchers and students. Each 500 gm pack comes with proper documentation to ensure traceability for your lab records.
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Applications in Research
1. Protein Crystallization: PEG 6000 is commonly used in crystallization screens to help proteins form ordered crystals suitable for X-ray diffraction studies.
2. PCR Optimization: Adding PEG 6000 to PCR mixtures can enhance reaction efficiency, especially for difficult templates or GC-rich sequences.
3. Cell Fusion: In hybridoma technology, PEG 6000 promotes the fusion of myeloma cells with antibody-producing B cells to create monoclonal antibody-producing cell lines.
4. DNA Precipitation: Used alongside salts to precipitate DNA from aqueous solutions during purification protocols.
5. Protein Pegylation: Serves as a starting material for creating PEGylated proteins with improved pharmacokinetic properties.
Handling and Storage
Store PEG 6000 in a cool, dry place away from direct sunlight. Keep the container tightly sealed when not in use to prevent moisture absorption. While generally considered low-hazard, always follow standard lab safety practices when handling chemical reagents.
Why Researchers Trust CDH PEG 6000
The CDH brand has built a strong reputation in Indian laboratories for providing consistent, high-purity reagents. This molecular biology grade PEG 6000 undergoes rigorous quality control to ensure: – Low endotoxin levels – Minimal heavy metal contamination – Consistent molecular weight distribution – Batch-to-batch reproducibility
For students and early-career researchers, using reliable reagents like this CDH PEG 6000 means fewer experimental variables to troubleshoot, leading to more reproducible results in your work.
FAQs
What is the molecular weight range of PEG 6000?
PEG 6000 typically has an average molecular weight between 5,400-7,000 Daltons, with most molecules falling within this range.
Can I use this PEG 6000 for cell culture applications?
While primarily designed for molecular biology applications, this grade can be used in cell culture at appropriate concentrations, though cell culture-tested grades are available for more sensitive applications.
How should I prepare a 20% PEG 6000 solution?
Dissolve 20 grams of PEG 6000 in 80 ml of distilled water, then adjust the final volume to 100 ml. Warm water (37-50°C) helps dissolution.
What’s the shelf life of unopened PEG 6000?
When stored properly in its original packaging, PEG 6000 typically has a shelf life of 3-5 years from the date of manufacture.
Where can I buy authentic CDH PEG 6000 in India?
You can purchase genuine CDH Polyethylene Glycol 6000 online at eqipped with assured quality and pan-India delivery.
